CD11b Antikörper (FITC)

Produktnummer ABIN6657852

Hersteller Produkt- Nr.: 200-502-n79

Dieser Ratte Monoklonal Antikörper detektiert spezifisch CD11b in FACS, IHC und IP. Es zeigt Reaktivität gegenüber Proben von Maus.

Kurzübersicht für CD11b Antikörper (FITC) (ABIN6657852)

Target: CD11b (ITGAM) (Integrin alpha M (ITGAM))

Reaktivität: Maus

Wirt: Ratte

Klonalität: Monoklonal

Konjugat: Dieser CD11b Antikörper ist konjugiert mit FITC

Applikation: Flow Cytometry (FACS), Immunohistochemistry (IHC), Immunoprecipitation (IP)

Klon: M1-70

Produktdetails anti-CD11b Antikörper (FITC)

Hersteller Produkt- Nr.: 200-502-n79

Hersteller: Rockland

Verwendungszweck: CD11b Fluorescein Antibody

Kreuzreaktivität (Details): Cross reactive to Human, Chimpanzee, Baboon, Cynomolgus, and Rhesus.

Aufreinigung: Fluorescein conjugated CD11b Monoclonal Antibody was purified from tissue culture supernatant via affinity chromatography and is directed against mouse CD11b.

Sterilität: Sterile filtered

Immunogen: Anti-CD11b Antibody (Monoclonal) was produced by repeated immunizations with CD11b antigen.

Isotyp: IgG2b kappa

Labeling Ratio: 2-8

Anwendungsinformationen

Applikationshinweise:

Immunoprecipitation_Dilution: User Optimized

Immunohistochemistry_Dilution: User Optimized

Flow_Cytometry_Dilution: 10 μL/10^6 cells (0.1 μg)

Other: Sufficient to run approximately 500 tests

Kommentare:

Anti-CD11b is tested for FLOW and useful for Immunoprecipitation and Immunohistochemistry using mouse spleen cells, or an appropriate cell type (where indicated). Researchers should determine optimal titers for applications that are not stated.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Buffer:

Buffer: 0.01 M Sodium Phosphate, 0.15 M Sodium Chloride, pH 7.2

Stabilizer: 0.1 % Gelatin

Preservative: 0.09 % (w/v) Sodium Azide

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Lagerung: 4 °C

Informationen zur Lagerung: Store vial at 4° C prior to opening.  This product is stable for several weeks at 4° C as an undiluted liquid.  Dilute only prior to immediate use.  DO NOT FREEZE. This product is light sensitive.

Haltbarkeit: 6 months

Details zu CD11b

Target: CD11b (ITGAM) (Integrin alpha M (ITGAM))

Andere Bezeichnung: CD11b

Hintergrund:

Synonyms: CD11b

Background: CD11b antibody reacts with human and mouse CD11b, also known as integrin alpha M. This 165-170 kDa cell surface glycoprotein is part of a family of integrin receptors that mediate adhesion between cells (cell-cell) and components of the extracellular matrix, e.g. fibrinogen (cell-matrix). In addition, integrins are active signaling receptors which recruit leukocytes to inflammatory sites and promote cell activation. Complete, functional integrin receptors consist of distinct combinations of integrin chains which are differentially expressed. Integrin alpha M (CD11b) assembles with Integrin beta-2 (CD18) into a receptor known as Macrophage Antigen-1 (Mac-1) or complement receptor type 3 (CR3). This receptor binds and induces intracellular signaling through ICAM-1 on endothelial cells and can also facilitate removal of iC3b bearing foreign cells.The M1/70 antibody is widely used as a marker for CD11b expression on mouse macrophages, granulocytes, neutrophils, and NK cells.

Gene Name: Itgam

UniProt: A1E2I0

Pathways: Apoptose, Activation of Innate immune Response, Toll-Like Receptors Cascades, Activated T Cell Proliferation