ERK1 Antikörper (Internal Region)

Produktnummer ABIN6657518

Hersteller Produkt- Nr.: 600-401-r20

Der Kaninchen Polyklonal anti-ERK1 Antikörper (ABIN6657518) detektiert spezifisch ERK1 in WB und IHC. Dieser Antikörper reagiert spezifisch mit Proben aus Human.

Kurzübersicht für ERK1 Antikörper (Internal Region) (ABIN6657518)

Target: ERK1 (MAPK3) (Mitogen-Activated Protein Kinase 3 (MAPK3))

Reaktivität: Human

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Dieser ERK1 Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), Immunohistochemistry (IHC)

Produktdetails anti-ERK1 Antikörper

Bindungsspezifität: Internal Region

Hersteller Produkt- Nr.: 600-401-r20

Hersteller: Rockland

Verwendungszweck: MAPK3 Antibody

Kreuzreaktivität (Details): Anti-MAPK3 antibody is directed against human MAPK3 protein.

Aufreinigung: The product was affinity purified from monospecific antiserum by immunoaffinity purification.

Immunogen: MAPK3 affinity purified antibody was prepared from whole rabbit serum produced by repeated immunizations with a synthetic peptide corresponding to an internal region of human MAPK3.

Isotyp: IgG

Anwendungsinformationen

Applikationshinweise:

Immunohistochemistry_Dilution: 0.5-1 μg/mL

Western_Blot_Dilution: 0.1-0.5 μg/mL

Other: Immunocytochemistry: 0.5-1 μg/mL

Kommentare:

Anti-MAPK3 is tested for Immunohistochemistry -P, Immunocytochemistry, and Western Blotting. Specific conditions for reactivity should be optimized by the end user. Expect a band approximately ~43.1 kDa corresponding to the appropriate cell lysate or extract.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Lyophilized

Rekonstitution:

Reconstitution_Buffer: Restore with deionized water (or equivalent)

Reconstitution_Volume: 100 μL

Buffer:

Buffer: 0.9 mg NaCl, 0.2 mg Na2HPO4, 0.05 mg Sodium azide

Stabilizer: 5 mg BSA

Preservative: 0.05 mg Thimerosal

Konservierungsmittel: Thimerosal (Merthiolate)

Vorsichtsmaßnahmen: This product contains Thimerosal (Merthiolate): a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Lagerung: 4 °C,-20 °C

Informationen zur Lagerung: Store vial at 4° C prior to opening. Aliquot contents and freeze at -20° C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.

Haltbarkeit: 12 months

Details zu ERK1

Target: ERK1 (MAPK3) (Mitogen-Activated Protein Kinase 3 (MAPK3))

Andere Bezeichnung: MAPK3

Hintergrund:

Synonyms: ERT2,Extracellular signal-regulated kinase 1,

Background: Mitogen-activated protein kinase 3 is an enzyme that in humans is encoded by the MAPK3 gene.The protein encoded by this gene is a member of the MAP kinase family. MAP kinases, also known as extracellular signal-regulated kinases (ERKs), act in a signaling cascade that regulates various cellular processes such as proliferation, differentiation, and cell cycle progression in response to a variety of extracellular signals. This kinase is activated by upstream kinases, resulting in its translocation to the nucleus where it phosphorylates nuclear targets. Alternatively spliced transcript variants encoding different protein isoforms have been described. MAPK3 gene is mapped to human chromosome 16 by hybrid cell panel analysis. This antibody is suitable for researchers interested in stem cell, neuroscience and cancer research.

Gene Name: MAPK3

Gen-ID: 5595

NCBI Accession: NP_001035145

UniProt: P27361

Pathways: MAPK Signalweg, RTK Signalweg, Interferon-gamma Pathway, Fc-epsilon Rezeptor Signalübertragung, Neurotrophin Signalübertragung, Response to Growth Hormone Stimulus, Activation of Innate immune Response, Cellular Response to Molecule of Bacterial Origin, Hepatitis C, Protein targeting to Nucleus, Toll-Like Receptors Cascades, Signaling Events mediated by VEGFR1 and VEGFR2, Signaling of Hepatocyte Growth Factor Receptor, VEGFR1 Specific Signals, S100 Proteine