Caspase 8 Antikörper

Produktnummer ABIN6655461

Hersteller Produkt- Nr.: 200-301-h64

Dieses Maus Monoklonal-Antikörper erkennt spezifisch Caspase 8 in WB, IHC und FACS. Er zeigt eine Reaktivität gegenüber Human.

Kurzübersicht für Caspase 8 Antikörper (ABIN6655461)

Target: Caspase 8 (CASP8)

Reaktivität: Human

Wirt: Maus

Klonalität: Monoklonal

Konjugat: Dieser Caspase 8 Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), Immunohistochemistry (IHC), Flow Cytometry (FACS)

Klon: 90A992

Produktdetails anti-Caspase 8 Antikörper

Hersteller Produkt- Nr.: 200-301-h64

Hersteller: Rockland

Verwendungszweck: Caspase-8 Antibody

Kreuzreaktivität (Details): A BLAST analysis was used to suggest cross-reactivity with Anti-Caspase-8 from Chimpanzee, Human, Rhesus Monkey and Mouse based on 100 % homology with the immunizing sequence.

Aufreinigung: Anti-Caspase-8 Antibody was purified by Protein G chromatography.

Immunogen: Caspase-8 Antibody was produced in mice prepared by repeated immunizations with amino acids at the internal portion of human Caspase-8.

Isotyp: IgG1

Anwendungsinformationen

Applikationshinweise:

Immunohistochemistry_Dilution: 5 μg/mL

Flow_Cytometry_Dilution: 0.1-0.5 μg/mL

Western_Blot_Dilution: 0.5-2 μg/mL

Kommentare:

Anti-Caspase-8 antibody is tested for use by WB, Flow, and IHC-P. Expect a band approximately 55 kDa on specific lysates. Specific conditions for reactivity should be optimized by the end user.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Buffer:

Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2

Stabilizer: 0.05 % BSA

Preservative: 0.05 % (w/v) Sodium Azide

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Lagerung: 4 °C,-20 °C

Informationen zur Lagerung: Store vial at -20° C prior to opening. Aliquot contents and freeze at -20° C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.

Haltbarkeit: 12 months

Details zu Caspase 8

Target: Caspase 8 (CASP8)

Andere Bezeichnung: Caspase-8

Hintergrund:

Synonyms: MCH5Caspase-8, CASP-8, CASP8, 3.4.22.61, Apoptotic cysteine protease, Apoptotic protease Mch-5, CAP4, FADD-homologous ICE/ced-3-like protease, FADD-like ICE, FLICE, ICE-like apoptotic protease 5, MORT1-associated ced-3 homolog, MACH, Caspase-8 subunit p18, Caspase-8 subunit p10

Background: Anti-Caspase-8 detects human Caspase. Caspases are a family of cysteine proteases that are key mediators of programmed cell death or apoptosis. The precursor form of all caspases is composed of a prodomain, and large and small catalytic subunits. The active forms of caspases are generated by several stimuli including ligand-receptor interactions, growth factor deprivation and inhibitors of cellular functions. All known caspases require cleavage adjacent to aspartates to liberate one large and one small subunit, which associate into a2b2 tetramer to form the active enzyme. Caspase-8 (FLICE) forms a direct link between the activation of CD95 and the caspase pathway. Overexpression of Caspase-8 induces apoptosis, which can be blocked by inhibitors specific for the ICE family. Anti-Caspase-8 antibody is ideal for investigators involved in apoptosis research.

Gene Name: Casp8

Gen-ID: 841

NCBI Accession: NP_001073593

UniProt: Q14790

Pathways: Apoptose, Caspase Kaskade in der Apoptose, TLR Signalweg, Activation of Innate immune Response, Tube Formation, Positive Regulation of Endopeptidase Activity, Toll-Like Receptors Cascades