CD11b Antikörper (PE)

Produktnummer ABIN6655311

Hersteller Produkt- Nr.: 200-308-n20

Dieser Maus Monoklonal Antikörper detektiert spezifisch CD11b in FACS, IP und FM. Es zeigt Reaktivität gegenüber Proben von Human.

Kurzübersicht für CD11b Antikörper (PE) (ABIN6655311)

Target: CD11b (ITGAM) (Integrin alpha M (ITGAM))

Reaktivität: Human

Wirt: Maus

Klonalität: Monoklonal

Konjugat: Dieser CD11b Antikörper ist konjugiert mit PE

Applikation: Flow Cytometry (FACS), Immunoprecipitation (IP), Fluorescence Microscopy (FM)

Klon: ICRF44

Produktdetails anti-CD11b Antikörper (PE)

Hersteller Produkt- Nr.: 200-308-n20

Hersteller: Rockland

Verwendungszweck: CD11b Phycoerythrin Antibody

Kreuzreaktivität (Details): Reactivity is observed against human CD11b, Baboon, Chimpanzee, Cynomolgus, Rhesus, and Swine.

Aufreinigung: Phycoerythrin conjugated CD11b Monoclonal Antibody was purified from tissue culture supernatant via affinity chromatography and is directed against human CD11b.

Sterilität: Sterile filtered

Immunogen: Anti-CD11b Antibody (Monoclonal) was produced by repeated immunizations with CD11b antigen.

Isotyp: IgG1 kappa

Labeling Ratio: 1-2

Anwendungsinformationen

Applikationshinweise:

Immunoprecipitation_Dilution: User Optimized

Flow_Cytometry_Dilution: 5μL/test/1x10e5 to 1x10e8 cells

IF_Microscopy_Dilution: User Optimized

Kommentare:

Anti-CD11b is tested for Flow Cytometry and is useful in Immunofluorescence and Immunoprecipitation. Researchers should determine optimal titers for applications that are not stated.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Buffer:

Buffer: 0.01 M Sodium Phosphate, 0.15 M Sodium Chloride, pH 7.2

Stabilizer: 0.1 % Gelatin

Preservative: 0.09 % (w/v) Sodium Azide

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Lagerung: 4 °C

Informationen zur Lagerung: Store vial at 4° C prior to opening. Dilute only prior to immediate use. This product is stable at 4° C as an undiluted liquid. Use subdued lighting during handling and incubation of cells prior to analysis. Store reagent in the dark. DO NOT FREEZE.

Haltbarkeit: 6 months

Details zu CD11b

Target: CD11b (ITGAM) (Integrin alpha M (ITGAM))

Andere Bezeichnung: CD11b

Hintergrund:

Synonyms: Integrin alpha-M, CD11 antigen-like family member B, CR-3 alpha chain, Cell surface glycoprotein MAC-1 subunit alpha, Leukocyte adhesion receptor MO1, Neutrophil adherence receptor, CD11b, CR3A

Background: The ICRF44 antibody reacts with human CD11b, also known as integrin alpha M. This 165-170 kDa cell surface glycoprotein is part of a family of integrin receptors that mediate adhesion between cells (cell-cell) and components of the extracellular matrix, e.g. fibrinogen (cell-matrix). In addition, integrins are active signaling receptors which recruit leukocytes to inflammatory sites and promote cell activation. Complete, functional integrin receptors consist of distinct combinations of integrin chains which are differentially expressed. Integrin alpha M (CD11b) assembles with Integrin beta-2 (CD18) into a receptor known as Macrophage Antigen-1 (Mac-1) or complement receptor type 3 (CR3). This receptor binds and induces intracellular signaling through ICAM-1, ICAM-2, ICAM-3 and ICAM-4 on endothelial cells and can also facilitate removal of iC3b bearing foreign cells.The ICRF44 antibody is widely used as a marker for CD11b expression on macrophages, granulocytes, and subsets of NK cells.

Gene Name: ITGAM

Gen-ID: 3684

NCBI Accession: NP_000623

UniProt: P11215

Pathways: Apoptose, Activation of Innate immune Response, Toll-Like Receptors Cascades, Activated T Cell Proliferation