p21 Antikörper (pThr145)
Kurzübersicht für p21 Antikörper (pThr145) (ABIN6256478)
Target
Alle p21 (CDKN1A) Antikörper anzeigenReaktivität
Wirt
Klonalität
Konjugat
Applikation
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Bindungsspezifität
- pThr145
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Spezifität
- Phospho-p21 Cip1 (Thr145) Antibody detects endogenous levels of p21 Cip1 only when phosphorylated at Threonine 145.
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Homologie
- Bovine,Horse,Sheep,Rabbit,Dog
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Aufreinigung
- The antibody is from purified rabbit serum by affinity purification via sequential chromatography on phospho- and non-phospho-peptide affinity columns.
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Immunogen
- A synthesized peptide derived from human p21 Cip1 around the phosphorylation site of Thr145.
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Isotyp
- IgG
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Applikationshinweise
- WB 1:500-1:2000, IHC 1:50-1:200, IF/ICC 1:100-1:500, ELISA(peptide) 1:20000-1:40000
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Beschränkungen
- Nur für Forschungszwecke einsetzbar
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Format
- Liquid
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Konzentration
- 1 mg/mL
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Buffer
- Rabbit IgG in phosphate buffered saline , pH 7.4, 150 mM NaCl, 0.02 % sodium azide and 50 % glycerol.
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Konservierungsmittel
- Sodium azide
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Vorsichtsmaßnahmen
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Lagerung
- -20 °C
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Informationen zur Lagerung
- Store at -20 °C. Stable for 12 months from date of receipt.
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Haltbarkeit
- 12 months
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- p21 (CDKN1A) (Cyclin-Dependent Kinase Inhibitor 1A (p21, Cip1) (CDKN1A))
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Andere Bezeichnung
- CDKN1A
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Hintergrund
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Description: May be involved in p53/TP53 mediated inhibition of cellular proliferation in response to DNA damage. Binds to and inhibits cyclin-dependent kinase activity, preventing phosphorylation of critical cyclin-dependent kinase substrates and blocking cell cycle progression. Functions in the nuclear localization and assembly of cyclin D-CDK4 complex and promotes its kinase activity towards RB1. At higher stoichiometric ratios, inhibits the kinase activity of the cyclin D-CDK4 complex. Inhibits DNA synthesis by DNA polymerase delta by competing with POLD3 for PCNA binding (PubMed:11595739).
Gene: CDKN1A
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Molekulargewicht
- kDa
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Gen-ID
- 1026
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UniProt
- P38936
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Pathways
- p53 Signalweg, PI3K-Akt Signalweg, Zellzyklus, AMPK Signaling, Fc-epsilon Rezeptor Signalübertragung, EGFR Signaling Pathway, Neurotrophin Signalübertragung, Mitotic G1-G1/S Phases, DNA Replication, Hepatitis C, Synthesis of DNA, Autophagie
Target
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