ULK1 Antikörper (pSer317)

Produktnummer ABIN6255203

Dieser Kaninchen Polyklonal Antikörper detektiert spezifisch ULK1 in WB, IF, IHC und ICC. Es zeigt Reaktivität gegenüber Proben von Human, Maus und Ratte.

Kurzübersicht für ULK1 Antikörper (pSer317) (ABIN6255203)

Target: ULK1 (Unc-51 Like Kinase 1 (ULK1))

Reaktivität: Human, Maus, Ratte

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Dieser ULK1 Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), Immunofluorescence (IF), Immunohistochemistry (IHC), Immunocytochemistry (ICC)

Produktdetails anti-ULK1 Antikörper

Bindungsspezifität: pSer317

Verwendungszweck: Phospho-ULK1 (Ser317) Antibody

Spezifität: Phospho-ULK(Ser317) Antibody detects endogenous levels of ULK.

Homologie: Pig,Bovine,Horse,Sheep,Rabbit,Dog,Chicken,Xenopus

Aufreinigung: The antibody is from purified rabbit serum by affinity purification via sequential chromatography on phospho-peptide and non-phospho-peptide affinity columns.

Immunogen: A synthesized peptide derived from human ULK around the phosphorylation site of Ser317.

Isotyp: IgG

Anwendungsinformationen

Applikationshinweise: WB 1:500-1:2000, IHC 1:50-1:200, IF/ICC 1:100-1:500

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Konzentration: 1 mg/mL

Buffer: Rabbit IgG in phosphate buffered saline , pH 7.4, 150 mM NaCl, 0.02 % sodium azide and 50 % glycerol.

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Lagerung: -20 °C

Informationen zur Lagerung: Store at -20 °C. Stable for 12 months from date of receipt.

Haltbarkeit: 12 months

Details zu ULK1

Target: ULK1 (Unc-51 Like Kinase 1 (ULK1))

Andere Bezeichnung: ULK1

Hintergrund:

Description: Serine/threonine-protein kinase involved in autophagy in response to starvation. Acts upstream of phosphatidylinositol 3-kinase PIK3C3 to regulate the formation of autophagophores, the precursors of autophagosomes. Part of regulatory feedback loops in autophagy: acts both as a downstream effector and negative regulator of mammalian target of rapamycin complex 1 (mTORC1) via interaction with RPTOR. Activated via phosphorylation by AMPK and also acts as a regulator of AMPK by mediating phosphorylation of AMPK subunits PRKAA1, PRKAB2 and PRKAG1, leading to negatively regulate AMPK activity. May phosphorylate ATG13/KIAA0652 and RPTOR, however such data need additional evidences. Plays a role early in neuronal differentiation and is required for granule cell axon formation. May also phosphorylate SESN2 and SQSTM1 to regulate autophagy (PubMed:25040165).

Gene: ULK1

Molekulargewicht: 140-150 kDa

Gen-ID: 8408

UniProt: O75385

Pathways: Regulation of Cell Size, Autophagie