Cyclin A Antikörper

Produktnummer ABIN6251854

Der Maus Monoklonal Anti-Cyclin A-Antikörper wurde für WB, IF und FACS validiert. Er ist geeignet, Cyclin A in Proben von Human, Maus, Rind (Kuh), Green Monkey und Nerz zu detektieren.

Kurzübersicht für Cyclin A Antikörper (ABIN6251854)

Target: Cyclin A (CCNA2) (Cyclin A2 (CCNA2))

Reaktivität: Human, Maus, Rind (Kuh), Green Monkey, Nerz

Wirt: Maus

Klonalität: Monoklonal

Konjugat: Dieser Cyclin A Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), Immunofluorescence (IF), Flow Cytometry (FACS)

Klon: E67

Produktdetails anti-Cyclin A Antikörper

Aufreinigung: Protein G affinity chromatography

Immunogen: Full length bovine protein was used as the immunogen for this CCNA2 antibody.

Isotyp: IgG2a kappa

Anwendungsinformationen

Applikationshinweise: Flow Cytometry: 0.5-1 μg/million cells

IF: 1-2 μg/mL





The concentration stated for each application is a general starting point. Variations in protocols, secondaries and substrates may require the Cyclin A2 antibody to be titered up or down for optimal performance.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Konzentration: 0.2 mg/mL

Buffer: PBS with 0.1 mg/mL BSA and 0.05 % sodium azide

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Lagerung: -20 °C

Informationen zur Lagerung: Aliquot and Store at -20C. Avoid freez-thaw cycles.

Details zu Cyclin A

Target: Cyclin A (CCNA2) (Cyclin A2 (CCNA2))

Andere Bezeichnung: Cyclin A2

Hintergrund: This antibody recognizes a protein of 50-55 kDa, which is identified as Cyclin A. Its epitope is located amino acids 144-148 of the human protein. Cyclins are regulatory subunits of the cyclin-dependent kinases (cdk's) and they control transition at different specific phases of the cell cycle. The temporal expression of cyclins is tightly regulated and subsequently plays a critical role in controlling the enzymatic activity of cdk's. These cyclin/cdk complexes are essential for passage through specific stages in the cell cycle. In mammalian somatic cells, Cyclin A is required for S-phase and passage through G2-phase. The D and E type cyclins regulate the passage of G1, while Cyclin B is a critical regulator of mitosis. Mutation or disruption of normal Cyclin A expression causes cells to arrest in G2-phase.

Gen-ID: 890

UniProt: P20248

Pathways: PI3K-Akt Signalweg, Zellzyklus, AMPK Signaling, Mitotic G1-G1/S Phases, DNA Replication, M Phase, Synthesis of DNA