Caspase 8 Antikörper (C-Term)

Produktnummer ABIN6243617

Dieses Maus Monoklonal-Antikörper erkennt spezifisch Caspase 8 in WB und FACS. Er zeigt eine Reaktivität gegenüber Human.

Kurzübersicht für Caspase 8 Antikörper (C-Term) (ABIN6243617)

Target: Caspase 8 (CASP8)

Reaktivität: Human

Wirt: Maus

Klonalität: Monoklonal

Konjugat: Dieser Caspase 8 Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), Flow Cytometry (FACS)

Klon: 550CT8-5-2

Produktdetails anti-Caspase 8 Antikörper

Bindungsspezifität: AA 427-461, C-Term

Aufreinigung: This antibody is purified through a protein G column, followed by dialysis against PBS.

Immunogen: This CASP8 antibody is generated from a mouse immunized with a KLH conjugated synthetic peptide between 427-461 amino acids from the C-terminal region of human CASP8.

Isotyp: IgG1

Anwendungsinformationen

Applikationshinweise: WB: 1:2000. FC: 1:25

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Buffer: Purified monoclonal antibody supplied in PBS with 0.09 % (W/V) sodium azide.

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Lagerung: 4 °C,-20 °C

Haltbarkeit: 6 months

Details zu Caspase 8

Target: Caspase 8 (CASP8)

Andere Bezeichnung: CASP8

Hintergrund: Most upstream protease of the activation cascade of caspases responsible for the TNFRSF6/FAS mediated and TNFRSF1A induced cell death. Binding to the adapter molecule FADD recruits it to either receptor. The resulting aggregate called death- inducing signaling complex (DISC) performs CASP8 proteolytic activation. The active dimeric enzyme is then liberated from the DISC and free to activate downstream apoptotic proteases. Proteolytic fragments of the N-terminal propeptide (termed CAP3, CAP5 and CAP6) are likely retained in the DISC. Cleaves and activates CASP3, CASP4, CASP6, CASP7, CASP9 and CASP10. May participate in the GZMB apoptotic pathways. Cleaves ADPRT. Hydrolyzes the small-molecule substrate, Ac-Asp-Glu-Val-Asp-|-AMC. Likely target for the cowpox virus CRMA death inhibitory protein. Isoform 5, isoform 6, isoform 7 and isoform 8 lack the catalytic site and may interfere with the pro-apoptotic activity of the complex.

Molekulargewicht: 55391

UniProt: Q14790

Pathways: Apoptose, Caspase Kaskade in der Apoptose, TLR Signalweg, Activation of Innate immune Response, Tube Formation, Positive Regulation of Endopeptidase Activity, Toll-Like Receptors Cascades