CD40 Antikörper (CF®543)

Produktnummer ABIN6184906

Der Maus Monoklonal Anti-CD40-Antikörper wurde für FACS, WB, IHC und IF validiert. Er ist geeignet, CD40 in Proben von Human zu detektieren.

Kurzübersicht für CD40 Antikörper (CF®543) (ABIN6184906)

Target: CD40

Reaktivität: Human

Wirt: Maus

Klonalität: Monoklonal

Konjugat: Dieser CD40 Antikörper ist konjugiert mit CF®543

Applikation: Flow Cytometry (FACS), Western Blotting (WB), Immunohistochemistry (IHC), Immunofluorescence (IF)

Klon: C40-1605

Produktdetails anti-CD40 Antikörper (CF®543)

Verwendungszweck: Mouse Monoclonal anti-CD40 / TNFRSF5 (C40/1605), CF543 Conjugate

Produktmerkmale: CD40 is a receptor on antigen-presenting cells of the immune system and is essential for mediating a broad variety of immune and inflammatory responses including T cell-dependent immunoglobulin class switching, memory B cell development, and germinal center formation. AT-hook transcription factor AKNA is reported to coordinately regulate the expression of this receptor and its ligand, which may be important for homotypic cell interactions. Adaptor protein TNFR2 interacts with this receptor and serves as a mediator of the signal transduction. The interaction of this receptor and its ligand is found to be necessary for amyloid-beta-induced microglial activation, and thus is thought to be an early event in Alzheimer disease pathogenesis. CD40 is expressed on B-lymphocytes, follicular dendritic cells, bone marrow-derived dendritic cells, thymic epithelium, and interdigitating cells in the T-cell zones of secondary lymphoid organs. Primary antibodies are available purified, or with a selection of fluorescent CF® dyes and other labels. CF® dyes offer exceptional brightness and photostability. Note: Conjugates of blue fluorescent dyes like CF®405S and CF®405M are not recommended for detecting low abundance targets, because blue dyes have lower fluorescence and can give higher non-specific background than other dye colors.

Immunogen: Recombinant human full-length CD40 protein

Isotyp: IgG1 kappa

Anwendungsinformationen

Applikationshinweise: Immunohistology (formalin) 1-2 μg/mL

• Staining of formalin-fixed tissues requires boiling tissue sections in 10 mM Tris, 1 mM EDTA  pH 9.0 for 10-20 min followed by cooling at RT for 20 min
• Immunofluorescence 1-2 μg/mL Flow cytometry 0.5-1 μg/million cells
• Western blotting 0.5-1 μg/mL
• Optimal dilution for a specific application should be determined by user

Kommentare:

L-428 Cells. Tonsil, Spleen or Thymus

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Konzentration: 100 μg/mL

Buffer: PBS/0.1 % BSA/0.05 % azide

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handhabung: Protect from light

Details zu CD40

Target: CD40

Andere Bezeichnung: CD40 / TNFRSF5

Hintergrund: B-cell surface antigen CD40, Bp50, CD40, CD40L receptor, GP39, HIGM1, IGM, IMD3, p50, TBAM, TNF receptor superfamily member 5, TNFRSF5, TRAP

Molekulargewicht: 43 kDa

Gen-ID: 958, 472860

UniProt: P25942

Pathways: NF-kappaB Signalweg, Cellular Response to Molecule of Bacterial Origin, M Phase, Regulation of Leukocyte Mediated Immunity, Positive Regulation of Immune Effector Process, Production of Molecular Mediator of Immune Response, Cancer Immune Checkpoints