Cyclin A Antikörper (CF®594)

Produktnummer ABIN6157619

Dieses Anti-Cyclin A-Antikörper ist ein Maus Monoklonal-Antikörper zur Detektion von Cyclin A in IF und FACS. Geeignet für Human, Maus, Rind (Kuh), Green Monkey, Nerz und Affe.

Kurzübersicht für Cyclin A Antikörper (CF®594) (ABIN6157619)

Target: Cyclin A (CCNA2) (Cyclin A2 (CCNA2))

Reaktivität: Human, Maus, Rind (Kuh), Green Monkey, Nerz, Affe

Wirt: Maus

Klonalität: Monoklonal

Konjugat: Dieser Cyclin A Antikörper ist konjugiert mit CF®594

Applikation: Immunofluorescence (IF), Flow Cytometry (FACS)

Klon: E67

Produktdetails anti-Cyclin A Antikörper (CF®594)

Verwendungszweck: Mouse Monoclonal anti-CyclinA2 (E67), CF594 Conjugate

Produktmerkmale: This antibody recognizes a protein of 54 kDa, which is identified as cyclin A. Its epitope is located amino acids 144-148 of human Cyclin A2. Cyclins are regulatory subunits of the cyclin-dependent kinases (cdk's) and they control transition at different specific phases of the cell cycle. The temporal expression of cyclins is tightly regulated and subsequently plays a critical role in controlling the enzymatic activity of cdk's. These cyclin/cdk complexes are essential for passage through specific stages in the cell cycle. In mammalian somatic cells, cyclin A is required for S-phase and passage through G2-phase. The D and E type cyclins regulate the passage of G1, while cyclin B is a critical regulator of mitosis. Mutation or disruption of normal cyclin A expression causes cells to arrest in G2-phase. Primary antibodies are available purified, or with a selection of fluorescent CF® dyes and other labels. CF® dyes offer exceptional brightness and photostability. Note: Conjugates of blue fluorescent dyes like CF®405S and CF®405M are not recommended for detecting low abundance targets, because blue dyes have lower fluorescence and can give higher non-specific background than other dye colors.

Immunogen: Full length bovine Cyclin A protein

Isotyp: IgG2a kappa

Anwendungsinformationen

Applikationshinweise: Flow Cytometry 0.5-1 μg/million cells/0.1 mL

• Immunofluorescence 1-2 μg/mL
• Optimal dilution for a specific application should be determined by user

Kommentare:

K-562 and HeLa Cells

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Konzentration: 100 μg/mL

Buffer: PBS/0.1 % BSA/0.05 % azide

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handhabung: Protect from light

Details zu Cyclin A

Target: Cyclin A (CCNA2) (Cyclin A2 (CCNA2))

Andere Bezeichnung: CyclinA2

Hintergrund: CCN1, CCNA, CCNA2, CCNA2_HUMAN, Cyclin A2

Molekulargewicht: 54 kDa

Gen-ID: 890, 58974

UniProt: P20248

Pathways: PI3K-Akt Signalweg, Zellzyklus, AMPK Signaling, Mitotic G1-G1/S Phases, DNA Replication, M Phase, Synthesis of DNA