AMACR Antikörper (CF®405S)

Produktnummer ABIN6155467

Der Kaninchen Polyklonal Anti-AMACR-Antikörper wurde für IHC (f) validiert. Er ist geeignet, AMACR in Proben von Human zu detektieren.

Kurzübersicht für AMACR Antikörper (CF®405S) (ABIN6155467)

Target: AMACR (alpha-Methylacyl-CoA Racemase (AMACR))

Reaktivität: Human

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Dieser AMACR Antikörper ist konjugiert mit CF®405S

Applikation: Immunohistochemistry (Formalin-fixed Sections) (IHC (f))

Klon: PAb

Produktdetails anti-AMACR Antikörper (CF®405S)

Verwendungszweck: Rabbit Polyclonal anti-AMACR / p504S CF405S Conjugate

Produktmerkmale: This antibody recognizes a protein of 54 kDa, which is identified as AMACR, also known as p504S. It is an enzyme that is involved in bile acid biosynthesis and i²-oxidation of branched-chain fatty acids. AMACR is essential in lipid metabolism. It is expressed in cells of premalignant high-grade prostatic intraepithelial neoplasia (HGPIN) and prostate adenocarcinoma. The majority of the carcinoma cells show a distinct granular cytoplasmic staining reaction. AMACR is present at low or undetectable levels in glandular epithelial cells of normal prostate and benign prostatic hyperplasia. A spotty granular cytoplasmic staining is seen in a few cells of the benign glands. AMACR is expressed in normal liver (hepatocytes), kidney (tubular epithelial cells) and gall bladder (epithelial cells). Expression has also been found in lung (bronchial epithelial cells) and colon (colonic surface epithelium). AMACR expression can also be found in hepatocellular carcinoma and kidney carcinoma. Past studies have also shown that AMACR is expressed in various colon carcinomas (well, moderately and poorly differentiated) and overexpressed in prostate carcinoma. Primary antibodies are available purified, or with a selection of fluorescent CF® dyes and other labels. CF® dyes offer exceptional brightness and photostability. Note: Conjugates of blue fluorescent dyes like CF®405S and CF®405M are not recommended for detecting low abundance targets, because blue dyes have lower fluorescence and can give higher non-specific background than other dye colors.

Immunogen: A synthetic peptide from human AMACR protein

Isotyp: IgG

Anwendungsinformationen

Applikationshinweise: Immunohistology formalin-fixed 1:50-1:100

• Staining of formalin-fixed tissues requires boiling tissue sections in 10 mM Tris with 1 mM EDTA,  pH 9.0, for 10-20 min followed by cooling at RT for 20 minutes
• Optimal dilution for a specific application should be determined by user

Kommentare:

HEK cells or Prostate Adenocarcinoma

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Konzentration: 100 μg/mL

Buffer: PBS/0.1 % BSA/0.05 % azide

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handhabung: Protect from light

Details zu AMACR

Target: AMACR (alpha-Methylacyl-CoA Racemase (AMACR))

Andere Bezeichnung: AMACR / p504S

Hintergrund: Alpha-methylacyl-CoA Racemase, CBAS4, Da1-8, Macr1, RACE, RM

Molekulargewicht: 54 kDa

Gen-ID: 23600, 508343

UniProt: Q9UHK6

Pathways: Monocarboxylic Acid Catabolic Process