NF-kB p65 Antikörper (C-Term)

Produktnummer ABIN576233

Der Kaninchen Polyklonal anti-NF-kB p65 Antikörper wird verwendet zum Nachweis von NF-kB p65 in Proben von Human, Maus und Ratte. Er wurde validiert für WB, ELISA, IP und EMSA.

Kurzübersicht für NF-kB p65 Antikörper (C-Term) (ABIN576233)

Target: NF-kB p65 (NFkBP65) (Nuclear Factor-kB p65 (NFkBP65))

Reaktivität: Human, Maus, Ratte

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Dieser NF-kB p65 Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), ELISA, Immunoprecipitation (IP), Electrophoretic Mobility-Shift Assay (EMSA)

Produktdetails anti-NF-kB p65 Antikörper

Bindungsspezifität: C-Term

Spezifität: Recognizes the human Transcription factor p65, also known as Nuclear factor NF-kappa-B p65 subunit, NFkappaB p65, RelA or Nuclear factor of kappa light polypeptide gene enhancer in B-cells 3. NFkappaB p65 is a 551 amino acid ~65 kD subunit of the NFkappaB transcription factor present in most cell types and is involved as the endpoint in an array of signal transduction events related to many processes including apotosis, immunity, inflammation and tumorigenesis. The most abundant form of NFkappaB appears to be the p65-p50 heterodimer. In the active form NFkappaB p65 is found in the nucleus co-localizing with the ATP-dependent RNA helicase. NFkappaB p65 is also found in an inactive form in the cytoplasm associated with the inhibitor IkappaB In a gel supershift assaywas found to be active against all p65 containing human, mouse or rat NFkappaB complexes using 0.5 μL to 1.0 μL per assay. The binding ofto the NFkappaB p65 subunit can be blocked using the NFkappaB p65 control peptide.

Immunogen: NFkB p65 peptide corresponding to the C-terminus region of the human protein conjugated to KLH.

Type of Immunogen: Synthetic peptide

Isotyp: IgG

Anwendungsinformationen

Applikationshinweise: Approved: ELISA (1:5000 - 1:25000), GS, IP, WB (1:2000 - 1:5000)

Kommentare:

Target Species of Antibody: Human

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Konzentration: Lot specific

Buffer: Serum, 0.01 % sodium azide.

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Details zu NF-kB p65

Target: NF-kB p65 (NFkBP65) (Nuclear Factor-kB p65 (NFkBP65))

Andere Bezeichnung: RELA / NFKB p65

Hintergrund: Name/Gene ID: RELA

Synonyms: RELA, NF-kappa-B p65delta3, NFKB3, p65, Transcription factor p65

Gen-ID: 5970

Pathways: NF-kappaB Signalweg, RTK Signalweg, T-Zell Rezeptor Signalweg, TLR Signalweg, Fc-epsilon Rezeptor Signalübertragung, Neurotrophin Signalübertragung, Activation of Innate immune Response, Cellular Response to Molecule of Bacterial Origin, Hepatitis C, Toll-Like Receptors Cascades, S100 Proteine