CD11b Antikörper

Produktnummer ABIN573960

Produktdetails anti-CD11b Antikörper

Target: CD11b (ITGAM) (Integrin alpha M (ITGAM))

Reaktivität: Human, Maus, Kaninchen

Wirt: Ratte

Klonalität: Monoklonal

Konjugat: Dieser CD11b Antikörper ist unkonjugiert

Applikation: Flow Cytometry (FACS), Immunohistochemistry (IHC), Immunoprecipitation (IP), Immunohistochemistry (Frozen Sections) (IHC (fro)), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Marke: IHC-plus™

Spezifität: Recognizes the murine CD11b cell surface antigen (also known as the alpha M integrin chain or MAC-1), a differentiation antigen expressed by granulocytes, monocytes, NK cells and tissue macrophages. The expression of CD11b increases during monocyte maturation and expression levels vary on tissue macrophages. Peritoneal macrophages are reported to express higher levels of CD11b than splenic macrophages. Clone M1/70.15 has been reported to block iC3b binding to its receptor. This product is routinely tested in flow cytometry on mouse peritoneal macrophages.

Aufreinigung: Ion exchange chromatography

Immunogen: T cell enriched splenocytes from B10 mice.

Type of Immunogen: Cells

Klon: M1-70-15

Isotyp: IgG2b

Anwendungsinformationen

Applikationshinweise: Approved: Flo (1:50), IHC, IHC-Fr, IHC-P (10 μg/mL), IP

Usage: Immunohistochemistry: This antibody was validated for use in immunohistochemistry on a panel of formalin-fixed, paraffin-embedded (FFPE) mouse tissues after heat induced antigen retrieval in  pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for this antibody was determined to be 10 μg/mL.

Kommentare:

Target Species of Antibody: Mouse

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Konzentration: Lot specific

Buffer: PBS, 0.09 % sodium azide.

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handhabung: Avoid repeat freeze-thaw cycles.

Lagerung: 4 °C,-20 °C

Informationen zur Lagerung: Short term: 4°C. Long term: Store at -20°C. Avoid freeze-thaw cycles.

Details zu CD11b

Target: CD11b (ITGAM) (Integrin alpha M (ITGAM))

Andere Bezeichnung: ITGAM / CD11b (ITGAM Produkte)

Hintergrund: Name/Gene ID: ITGAM
Family: Integrin

Synonyms: ITGAM, Antigen CD11b (p170), CD11B, CD11b antigen, CR3A, Integrin alpha-M, MAC-1, MO1A, Neutrophil adherence receptor, MAC1A, SLEB6, CR-3 alpha chain

Gen-ID: 3684

UniProt: P11215

Pathways: Apoptose, Activation of Innate immune Response, Toll-Like Receptors Cascades, Activated T Cell Proliferation