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AIM2 Antikörper (C-Term)

Verified AIM2 Reaktivität: Human WB, ELISA, IHC, FACS, IF Wirt: Ziege Polyclonal unconjugated
Produktnummer ABIN571131
  • Target Alle AIM2 Antikörper anzeigen
    AIM2 (Absent in Melanoma 2 (AIM2))
    Bindungsspezifität
    • 16
    • 10
    • 8
    • 7
    • 5
    • 4
    • 3
    • 3
    • 2
    • 2
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    C-Term
    Reaktivität
    • 68
    • 24
    • 18
    • 3
    • 2
    • 1
    Human
    Wirt
    • 56
    • 12
    • 1
    Ziege
    Klonalität
    • 60
    • 9
    Polyklonal
    Konjugat
    • 38
    • 6
    • 5
    • 4
    • 3
    • 2
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    Dieser AIM2 Antikörper ist unkonjugiert
    Applikation
    • 62
    • 33
    • 32
    • 21
    • 13
    • 13
    • 12
    • 9
    • 6
    • 6
    • 3
    • 2
    • 2
    • 1
    Western Blotting (WB), ELISA, Immunohistochemistry (IHC), Flow Cytometry (FACS), Immunofluorescence (IF)
    Verwendungszweck
    AIM2
    Sequenz
    GEKLQLTSGV HSTIK
    Kreuzreaktivität
    Human
    Aufreinigung
    Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
    Güteklasse
    Verified
    Immunogen
    Peptide with sequence C-GEKLQLTSGVHSTIK, from the C Terminus of the protein sequence according to NP_004824.1.
    Isotyp
    IgG
    Top Product
    Discover our top product AIM2 Primärantikörper
  • Applikationshinweise

    Immunohistochemistry: In paraffin embedded Human Spleen shows nucleolar staining in some splenocytes. Recommended concentration: 4-6 μg/mL.

    Western Blot: Approx 37 kDa band observed in lysates of cell line HeLa (calculated MW of 39.0 kDa according to Human NP_004824.1). Recommended concentration: 0.5-1 μg/mL. Primary incubation 1 hour at room temperature.

    Peptide ELISA: antibody detection limit dilution 1:8000.

    Kommentare

    Immunofluorescence: Strong expression of the protein seen in U2OS and HeLa cells. Recommended concentration: 10μg/ml.

    Flow Cytometry: Flow cytometric analysis of HeLa cells. Recommended concentration: 10ug/ml.

    Beschränkungen
    Nur für Forschungszwecke einsetzbar
  • Format
    Liquid
    Konzentration
    0.5 mg/mL
    Buffer
    Supplied at 0.5 mg/mL in Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin.
    Konservierungsmittel
    Sodium azide
    Vorsichtsmaßnahmen
    This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
    Handhabung
    Minimize freezing and thawing.
    Lagerung
    -20 °C
    Informationen zur Lagerung
    Aliquot and store at -20°C, with minimal freeze/thawing. A working aliquot may be refrigerated at 4°C for a few weeks and still remain viable.
  • Target
    AIM2 (Absent in Melanoma 2 (AIM2))
    Andere Bezeichnung
    AIM2 (AIM2 Produkte)
    Synonyme
    AIM2 antikoerper, PYHIN4 antikoerper, Gm1313 antikoerper, Ifi210 antikoerper, absent in melanoma 2 antikoerper, AIM2 antikoerper, Aim2 antikoerper
    Hintergrund
    AIM2, absent in melanoma 2, PYHIN4
    Molekulargewicht
    39.0kDa
    Gen-ID
    9447
    NCBI Accession
    NP_004824
    Pathways
    Activation of Innate immune Response, Positive Regulation of Endopeptidase Activity, Inflammasome
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