CD14 Antikörper (AA 16-260)

Produktnummer ABIN5708067

Der Kaninchen Polyklonal Anti-CD14 Antikörper wurde für WB, IHC (p) und ELISA (Capture) validiert. Er ist geeignet, CD14 in Proben von Maus und Ratte zu detektieren.

Kurzübersicht für CD14 Antikörper (AA 16-260) (ABIN5708067)

Target: CD14

Reaktivität: Maus, Ratte

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Dieser CD14 Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), ELISA (Capture)

Produktdetails anti-CD14 Antikörper

Bindungsspezifität: AA 16-260

Aufreinigung: Antigen affinity purified

Immunogen: A recombinant mouse protein corresponding to amino acids S16-A260 was used as the immunogen for the CD14 antibody.

Isotyp: IgG

Anwendungsinformationen

Applikationshinweise: Optimal dilution of the CD14 antibody should be determined by the researcher.\. Western Blot: 0.5-1 μg/mL,IHC (FFPE): 1-2 μg/mL,ELISA (Capture, recombinant mouse protein): 0.1-0.5 μg/mL (BSA-free formulation available)

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Buffer: 0.5 mg/mL if reconstituted with 0.2 mL sterile DI water

Lagerung: -20 °C

Informationen zur Lagerung: After reconstitution, the CD14 antibody can be stored for up to one month at 4°C. For long-term, aliquot and store at -20°C. Avoid repeated freezing and thawing.

Details zu CD14

Target: CD14

Andere Bezeichnung: CD14

Hintergrund: CD14 is a single-copy gene encoding 2 protein forms: a 50- to 55 kDa glycosylphosphatidylinositol-anchored membrane protein (mCD14) and a monocyte or liver-derived soluble serum protein (sCD14) that lacks the anchor. This gene is located at bands 5q23-q31. The protein encoded by this gene is a surface antigen that is preferentially expressed on monocytes/macrophages. CD14 acts as a co-receptor (along with the Toll-like receptor TLR 4 and MD-2) for the detection of bacterial lipopolysaccharide (LPS). CD14 can bind LPS only in the presence of lipopolysaccharide-binding protein (LBP). Although LPS is considered its main ligand, CD14 also recognizes other pathogen-associated molecular patterns.

UniProt: P10810

Pathways: TLR Signalweg, Activation of Innate immune Response, Cellular Response to Molecule of Bacterial Origin, Toll-Like Receptors Cascades