TNF alpha Antikörper

Produktnummer ABIN5706833

Hersteller Produkt- Nr.: 110-401-321-0100

Der Kaninchen Polyklonal anti-TNF alpha Antikörper wird verwendet zum Nachweis von TNF alpha in Proben von Maus. Er wurde validiert für WB, IHC und ELISA.

Kurzübersicht für TNF alpha Antikörper (ABIN5706833)

Target: TNF alpha (Tumor Necrosis Factor alpha (TNF alpha))

Reaktivität: Maus

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Dieser TNF alpha Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), Immunohistochemistry (IHC), ELISA

Produktdetails anti-TNF alpha Antikörper

Hersteller Produkt- Nr.: 110-401-321-0100

Hersteller: Rockland

Verwendungszweck: TNF alpha Antibody

Kreuzreaktivität (Details): The antiserum is directed against mature 17,000 MW mouse TNFa and is useful in determining its presence in various assays.

Produktmerkmale: Synonyms: rabbit anti-Mouse TNF alpha Antibody, APC1 antibody, Cachectin antibody, DIF antibody, Differentiation inducing factor antibody, Macrophage cytotoxic factor antibody, MCF antibody, Necrosin antibody

Aufreinigung: This antiserum has been heated to 56°C for 30 minutes.

Sterilität: Sterile filtered

Immunogen:

Immunogen: The whole rabbit serum was prepared by repeated immunizations with recombinant mouse TNFa produced in E.coli.

Immunogen Type: Recombinant Protein

Anwendungsinformationen

Applikationshinweise:

Immunohistochemistry Dilution: 1:100 - 1:500

Application Note: This antiserum against Mouse TNFa has been tested for use in immunoblotting.  This antibody is suitable for use in neutralizations, ELISA, radioimmunoassay, immunoprecipitation, and immunohistochemistry. Reactivity in other immunoassays is unknown. It recognizes the 17,000 MW TNFa. This antiserum will recognize the cell-bound precursor of TNFa as a 26,000 protein in immunoblots, particularly in denatured samples.  This antibody is also useful for neutralization of mouse activity in bioassays.  It does not neutralize the biological activity of lymphotoxin.  For neutralization, it is recommended to incubate the sample with a 1:200 dilution of the antibody for at least 4 hours before being tested.  A control of similarly diluted normal rabbit IgG is recommended.

Western Blot Dilution: 1:500 - 1:2,000

ELISA Dilution: 1:1,000 - 1:5,000

Other: User Optimized

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Konzentration: 85 mg/mL

Buffer:

Buffer: None

Stabilizer: None

Preservative: None

Konservierungsmittel: Without preservative

Lagerung: 4 °C,-20 °C

Informationen zur Lagerung: Store vial at -20° C prior to opening. Aliquot contents and freeze at -20° C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.

Haltbarkeit: 12 months

Details zu TNF alpha

Target: TNF alpha (Tumor Necrosis Factor alpha (TNF alpha))

Andere Bezeichnung: Tnf

Hintergrund: Background: TNF alpha Antibody detects TNF-a protein. TNF-a is a cytokine that binds to TNFRSF1A/TNFR1 and TNFRSF1B/TNFBR. It is mainly secreted by macrophages and can induce cell death of certain tumor cell lines. It is a potent pyrogen causing fever by direct action or by stimulation of interleukin-1 secretion and is implicated in the induction of cachexia, Under certain conditions it can stimulate cell proliferation and induce cell differentiation. Anti-TNF-alpha Antibody is ideal for investigators involved in Cell Signaling, Immunology and Signal Transduction research.

Gen-ID: 21926

UniProt: P06804

Pathways: NF-kappaB Signalweg, Apoptose, Caspase Kaskade in der Apoptose, TLR Signalweg, Cellular Response to Molecule of Bacterial Origin, Regulation of Leukocyte Mediated Immunity, Positive Regulation of Immune Effector Process, Production of Molecular Mediator of Immune Response, Positive Regulation of Endopeptidase Activity, Hepatitis C, Protein targeting to Nucleus, Inflammasome