NF-kB p65 Antikörper (acLys310)

Produktnummer ABIN5706804

Hersteller Produkt- Nr.: 600-401-j88

Der Kaninchen Polyklonal anti-NF-kB p65 Antikörper (ABIN5706804) detektiert spezifisch NF-kB p65 in WB, IHC und ELISA. Dieser Antikörper reagiert spezifisch mit Proben aus Human, Maus, Ratte, Huhn und Primate.

Kurzübersicht für NF-kB p65 Antikörper (acLys310) (ABIN5706804)

Target: NF-kB p65 (NFkBP65) (Nuclear Factor-kB p65 (NFkBP65))

Reaktivität: Human, Maus, Ratte, Huhn, Primate

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Dieser NF-kB p65 Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), Immunohistochemistry (IHC), ELISA

Produktdetails anti-NF-kB p65 Antikörper

Bindungsspezifität: acLys310

Hersteller Produkt- Nr.: 600-401-j88

Hersteller: Rockland

Verwendungszweck: NFkB [ac Lys 310] Antibody

Kreuzreaktivität (Details): A BLAST analysis was used to suggest cross-reactivity with mouse and human based on 100 % sequence homology.

Produktmerkmale: Synonyms: rabbit anti-NFKB Ac Lys310 antibody, rabbit anti-NfKB K310ac antibody, NFKB, NFkß, NF-kB, NF-kappaB, NFkappaB, Transcription factor p65, Nuclear factor NF-kappa-B p65 subunit, Nuclear factor of kappa light polypeptide gene enhancer in B-cells 3, RELA, NFKB3

Aufreinigung: Anti-NFkB [ac Lys 310] Antibody was affinity purified from monospecific antiserum by immunoaffinity chromatography.

Sterilität: Sterile filtered

Immunogen:

Immunogen: Anti-NFkB K310ac affinity purified antibody was prepared from whole rabbit serum produced by repeated immunizations with a synthetic acetylated peptide surrounding Lysine 310 of human TF65 protein.

Immunogen Type: Conjugated Peptide

Isotyp: IgG

Anwendungsinformationen

Applikationshinweise:

Immunohistochemistry Dilution: User Optimized

Application Note: Anti-NFkB [ac Lys 310] Antibody has been tested in ELISA. Anti-NFkB K310ac is useful for in Immunohistochemistry and Western Blot. Specific conditions for reactivity should be optimized by the end user. Expect a band approximately ~65 kDa corresponding to the appropriate cell lysate or extract.

Western Blot Dilution: 1 μg/mL

ELISA Dilution: 1:50,000

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Konzentration: 1.05 mg/mL

Buffer:

Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2

Stabilizer: 50 % (v/v) Glycerol with 1 mg/mL Bovine Serum Albumin (BSA)

Preservative: None

Konservierungsmittel: Without preservative

Lagerung: 4 °C,-20 °C

Informationen zur Lagerung: Store vial at -20° C prior to opening. Aliquot contents and freeze at -20° C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.

Haltbarkeit: 12 months

Details zu NF-kB p65

Target: NF-kB p65 (NFkBP65) (Nuclear Factor-kB p65 (NFkBP65))

Andere Bezeichnung: RELA

Hintergrund: Background: NFkB is a pleiotropic transcription factor present in most cell types and is the endpoint of a series of signal transduction events related to many biological processes such as inflammation, immunity, differentiation, cell growth, tumorgenesis and apoptosis. NFkB is contained in the cytoplasm inactively until it is released by a phosphorylated I-kappa-B, allowing the NFkB to translocate to the nucleus. This antibody is ideal for investigators interested in Metabolism, Cancer, Cardiovascular, Microbiology, Epigenetics and Nuclear Signaling, Immunology, Neuroscience, or Cell Biology research.

UniProt: Q04206

Pathways: NF-kappaB Signalweg, RTK Signalweg, T-Zell Rezeptor Signalweg, TLR Signalweg, Fc-epsilon Rezeptor Signalübertragung, Neurotrophin Signalübertragung, Activation of Innate immune Response, Cellular Response to Molecule of Bacterial Origin, Hepatitis C, Toll-Like Receptors Cascades, S100 Proteine