CD11b Antikörper (AA 129-336)

Produktnummer ABIN5693079

Dieses Kaninchen Polyklonal-Antikörper erkennt spezifisch CD11b in FACS, WB und ELISA. Er zeigt eine Reaktivität gegenüber Human, Maus und Ratte und wurde in 4+ Publikationen erwähnt.

Kurzübersicht für CD11b Antikörper (AA 129-336) (ABIN5693079)

Target: CD11b (ITGAM) (Integrin alpha M (ITGAM))

Reaktivität: Human, Maus, Ratte

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Dieser CD11b Antikörper ist unkonjugiert

Applikation: Flow Cytometry (FACS), Western Blotting (WB), ELISA

Produktdetails anti-CD11b Antikörper

Bindungsspezifität: AA 129-336

Verwendungszweck: Anti-CD11b/ITGAM Antibody Picoband®

Kreuzreaktivität (Details): No cross-reactivity with other proteins.

Produktmerkmale: Anti-CD11b/ITGAM Antibody Picoband® (ABIN5693079). Tested in ELISA, Flow Cytometry, WB applications. This antibody reacts with Human, Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.

Immunogen: E. coli-derived human CD11b recombinant protein (Position: N129-E336).

Isotyp: IgG

Anwendungsinformationen

Applikationshinweise: Western blot, 0.1-0.5 μg/mL
Flow Cytometry (Fixed), 1-3 μg/1x10⁶ cells, Human
ELISA, 0.1-0.5 μg/mL
1. Arnaout, M. A., Remold-O'Donnell, E., Pierce, M. W., Harris, P., Tenen, D. G. Molecular cloning of the alpha-subunit of human and guinea pig leukocyte adhesion glycoprotein Mo1: chromosomal localization and homology to the alpha-subunits of integrins. Proc. Nat. Acad. Sci. 85: 2776-2780, 1988. 2. Solovjov D, Pluskota E, Plow E (2005). "Distinct roles for the alpha and beta subunits in the functions of integrin alphaMbeta2". J Biol Chem 280 (2): 1336-45. 3. Simon, D. I., Chen, Z., Seifert, P., Edelman, E. R., Ballantyne, C. M., Rogers, C. Decreased neointimal formation in Mac-1 -/- mice reveals a role for inflammation in vascular repair after angioplasty. J. Clin. Invest. 105: 293-300, 2000.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Lyophilized

Rekonstitution: Add 0.2 mL of distilled water will yield a concentration of 500 μg/mL.

Konzentration: 500 μg/mL

Buffer: Each vial contains 4 mg Trehalose, 0.9 mg NaCl and 0.2 mg Na2HPO4.

Lagerung: 4 °C,-20 °C

Informationen zur Lagerung: Store at -20°C for one year from date of receipt. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freeze-thaw cycles.

Referenzen für anti-CD11b Antikörper (ABIN5693079)

Mu, Jing, Guo: "Expressions of CD11a, CD11b, and CD11c integrin proteins in rats with myocardial hypertrophy." in: Iranian journal of basic medical sciences, Vol. 17, Issue 11, pp. 874-8, (2015) (PubMed).

Yuan, Su, Pu, Liu, Chen, Zhu, Zhu, Zhang, He: "Ethyl pyruvate promotes spinal cord repair by ameliorating the glial microenvironment." in: British journal of pharmacology, Vol. 166, Issue 2, pp. 749-63, (2012) (PubMed).

Leng, Zhan, Wang, Liu, Gong, Gao, Wu, Wang, Zhao, Wang, Zhang, Pang, Qian: "Anti-heat shock protein 70 autoantibody epitope changes and BD091 promotes atherosclerosis in rats." in: Cell stress & chaperones, Vol. 15, Issue 6, pp. 947-58, (2011) (PubMed).

Shi, Xun, Yue, Zhang, Ren, Wang, Wu, Shi, Yang, Lei: "Cloning, characterization, and expression analysis of goat (Capra hircus) phospholipid hydroperoxide glutathione peroxidase (PHGPx)." in: International journal of biological sciences, Vol. 6, Issue 4, pp. 316-26, (2010) (PubMed).

Details zu CD11b

Target: CD11b (ITGAM) (Integrin alpha M (ITGAM))

Andere Bezeichnung: ITGAM

Hintergrund:

Synonyms: Integrin alpha-M, CD11 antigen-like family member B, CR-3 alpha chain, Cell surface glycoprotein MAC-1 subunit alpha, Leukocyte adhesion receptor MO1, Neutrophil adherence receptor, CD11b, ITGAM, CD11B, CR3A

Tissue Specificity: Predominantly expressed in monocytes and granulocytes.

Background: Integrin alpha M (ITGAM) is one protein subunit that forms the heterodimeric integrin alpha-M beta-2 (αMβ2) molecule, also known as macrophage-1 antigen (Mac-1) or cluster of differentiation molecule 11B (CD11B). It is mapped to 16p11.2. ITGAM has a role in vascular repair after mechanical arterial injury. It is implicated in various adhesive interactions of monocytes, macrophages and granulocytes as well as in mediating the uptake of complement-coated particles. What's more, ITGAM probably recognizes the R-G-D peptide in C3b, and it is also a receptor for fibrinogen, factor X and ICAM1.

Molekulargewicht: 170 kDa

Gen-ID: 3684

UniProt: P11215

Pathways: Apoptose, Activation of Innate immune Response, Toll-Like Receptors Cascades, Activated T Cell Proliferation