IGF1 Antikörper

Produktnummer ABIN5689021

Der Kaninchen Polyklonal Anti-IGF1-Antikörper wurde für WB, ELISA und Neut validiert. Er ist geeignet, IGF1 in Proben von Human zu detektieren.

Kurzübersicht für IGF1 Antikörper (ABIN5689021)

Target: IGF1 (Insulin-Like Growth Factor 1 (IGF1))

Reaktivität: Human

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Dieser IGF1 Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), ELISA, Neutralization (Neut)

Produktdetails anti-IGF1 Antikörper

Aufreinigung: Anti-hIGF-1 specific antibody was purified by affinity chromatography employing immobilized hIGF-1 matrix.

Immunogen: Produced from sera of rabbits pre-immunized with highly pure (>98%) recombinant hIGF-1 (human Insulin Like Growth Factor-1).

Anwendungsinformationen

Applikationshinweise: Neutralization:
To yield one-half maximal inhibition [ND50] of the biological activity of hIGF-1 (3.00 ng/mL), a concentration of 0.67 0.1 μ,g/mL of this antibody is required.

ELISA:

To detect hIGF-1 by direct ELISA (using 100 μ,L/well antibody solution) a concentration of at least 0.5 μ,g/mL of this antibody is required. This antigen affinity purified antibody, in conjunction with compatible secondary reagents, allows the detection of 0.2 - 0.4 ng/well of recombinant hIGF-1.

Sandwich:

To detect hIGF-I by sandwich ELISA (using 100 μ,L/well antibody solution) a concentration of 0.5 - 2.0 μ,g/mL of this antibody is required. This antigen affinity purified antibody, in conjunction with our Biotinylated Anti-Human IGF-I (XP-5158Bt) as a detection antibody, allows the detection of at least 0.2 - 0.4 ng/well of recombinant hIGF-I.

Western Blot:

To detect hIGF-1 by Western Blot analysis this antibody can be used at a concentration of 0.1 - 0.2 μ,g/mL. Used in conjunction with compatible secondary reagents the detection limit for recombinant hIGF-1 is 1.5 - 3.0 ng/lane, under either reducing or non-reducing conditions.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Lyophilized

Lagerung: -20 °C

Informationen zur Lagerung: IGF-I antibody is stable for at least 2 years from date of receipt at -20°C. The reconstituted antibody is stable for at least two weeks at 2-8°C. Frozen aliquots are stable for at least 6 months when stored at -20°C. Avoid repeated freeze-thaw cycles.

Details zu IGF1

Target: IGF1 (Insulin-Like Growth Factor 1 (IGF1))

Andere Bezeichnung: IGF-I

Gen-ID: 3486

Pathways: RTK Signalweg, Intracellular Steroid Hormone Receptor Signaling Pathway, Peptide Hormone Metabolism, Hormone Activity, Regulation of Intracellular Steroid Hormone Receptor Signaling, Regulation of Hormone Metabolic Process, Regulation of Hormone Biosynthetic Process, Stem Cell Maintenance, Glycosaminoglycan Metabolic Process, Regulation of Carbohydrate Metabolic Process, Autophagie, Smooth Muscle Cell Migration, Activated T Cell Proliferation, Positive Regulation of fat Cell Differentiation