IGF1 Antikörper

Produktnummer ABIN5687643

Dieses Ziege Polyklonal-Antikörper erkennt spezifisch IGF1 in WB, ELISA und Neut. Er zeigt eine Reaktivität gegenüber Maus.

Kurzübersicht für IGF1 Antikörper (ABIN5687643)

Target: IGF1 (Insulin-Like Growth Factor 1 (IGF1))

Reaktivität: Maus

Wirt: Ziege

Klonalität: Polyklonal

Konjugat: Dieser IGF1 Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), ELISA, Neutralization (Neut)

Produktdetails anti-IGF1 Antikörper

Aufreinigung: Anti-murine IGF-I specific antibody was purified by affinity chromatography employing immobilized murine IGF-I matrix.

Immunogen: Produced from sera of goats pre-immunized with highly pure (>98%) recombinant murine IGF-I.

Anwendungsinformationen

Applikationshinweise: Neutralization:
To yield one-half maximal inhibition [ND50] of the biological activity of murine IGF-I (200 ng/mL), a concentration of 12-14 μ,g/mL of this antibody is required.

ELISA:

To detect murine IGF-I by direct ELISA (using 100 μ,L/well antibody solution) a concentration of at least 0.5 μ,g/mL of this antibody is required. This antigen affinity purified antibody, in conjunction with compatible secondary reagents, allows the detection of 0.2 - 0.4 ng/well of recombinant murine IGF-I.

Sandwich:

To detect mIGF-I by sandwich ELISA (using 100 μ,L/well antibody solution) a concentration of 0.5 - 2.0 μ,g/mL of this antibody is required. This antigen affinity purified antibody, in conjunction with our Biotinylated Anti-Murine IGF-I (XP-5159Bt) as a detection antibody, allows the detection of at least 0.2 - 0.4 ng/well of recombinant mIGF-I.

Western Blot:

To detect murine IGF-I by Western Blot analysis this antibody can be used at a concentration of 0.1 - 0.2 μ,g/mL. Used in conjunction with compatible secondary reagents the detection limit for recombinant murine IGF-I is 1.5 - 3.0 ng/lane, under either reducing or non-reducing conditions.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Lyophilized

Lagerung: -20 °C

Informationen zur Lagerung: IGF-I antibody is stable for at least 2 years from date of receipt at -20°C. The reconstituted antibody is stable for at least two weeks at 2-8°C. Frozen aliquots are stable for at least 6 months when stored at -20°C. Avoid repeated freeze-thaw cycles.

Details zu IGF1

Target: IGF1 (Insulin-Like Growth Factor 1 (IGF1))

Andere Bezeichnung: IGF-I

Gen-ID: 16000

UniProt: P05017

Pathways: RTK Signalweg, Intracellular Steroid Hormone Receptor Signaling Pathway, Peptide Hormone Metabolism, Hormone Activity, Regulation of Intracellular Steroid Hormone Receptor Signaling, Regulation of Hormone Metabolic Process, Regulation of Hormone Biosynthetic Process, Stem Cell Maintenance, Glycosaminoglycan Metabolic Process, Regulation of Carbohydrate Metabolic Process, Autophagie, Smooth Muscle Cell Migration, Activated T Cell Proliferation, Positive Regulation of fat Cell Differentiation