Collagen IV Antikörper

Produktnummer ABIN5680248

Dieser Kaninchen Polyklonal Antikörper erkennt spezifisch Collagen IV in WB, ELISA, IHC (p), IF und RIA. Er zeigt eine Reaktivität gegenüber Maus.

Kurzübersicht für Collagen IV Antikörper (ABIN5680248)

Target: Collagen IV (COL4) (Collagen, Type IV (COL4))

Reaktivität: Maus

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Dieser Collagen IV Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), ELISA, Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunofluorescence (IF), Radioimmunoassay (RIA)

Produktdetails anti-Collagen IV Antikörper

Spezifität: Murine collagen type IV 100 % , murine collagen type I, II and III <0.1 %, human collagen type IV and V <0.1 %, murine fibronectin <0.1 %, murine laminin <0.1 % in RIA at 1:5000 dilution

Kreuzreaktivität (Details): Mouse

Produktmerkmale: Type IV Collagen is a non-fibrillary network of different alpha-chains: alpha 1(IV) to-alpha 6(IV). It is typically found in basal membranes of different organs (e.g. skin, lens, lung, kidney). Collagens consist of a family of highly specialized glycoproteins of which at least 16 genetically distinct types are known to date. The basal unit of a collagen molecule consists of a triple-helical structure formed by 3 alpha-chains. Predominant amino acids are glycine, proline and hydroxproline. Regularly also lysines and hydroxylysines occur, which are responsible for cross-linkage and glycosylation of the protein chains. Different composition of alpha-chains and different glycosylation contribute to the high variability of collagens in different tissues and organs. Murine collagen type IV 100 % , murine collagen type I, II and III <0.1 %, human collagen type IV and V <0.1 %, murine fibronectin <0.1 %, murine laminin <0.1 % in RIA at 1:5000 dilution.

Aufreinigung: Affinity purified

Immunogen: Purified collagen type IV from murine tumour tissue

Isotyp: IgG

Anwendungsinformationen

Applikationshinweise: After de-waxing the tissue slices they are treated with 0.2 % hyaluronidase (app. 300 U/mg ) in TBS 15 min at 37 °C. Thereafter non-specific binding is blocked by blocking serum or 3 % BSA in TBS. For peroxidase systems blocking with 1 % peroxide solution in TBS for 30 min at RT is recommended.,Mouse skin or liver,IHC(P), IFA, ELISA, RIA, IB/WB,IHC(P) 60 min at RT or 2-8 °C over night,(purified, lyophilized) IFA ? 1:80, IHC(P) ? 1:500, ELISA ? 1:200 (OD ? 500)

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Lyophilized

Konzentration: 1 mg/mL

Buffer: phosphate buffered solution, no BSA and preservative added!

Konservierungsmittel: Without preservative

Lagerung: -20 °C

Details zu Collagen IV

Target: Collagen IV (COL4) (Collagen, Type IV (COL4))

Andere Bezeichnung: Collagen Type IV