Collagen IV Antikörper

Produktnummer ABIN5680248

Dieses Kaninchen Polyklonal-Antikörper erkennt spezifisch Collagen IV in WB, ELISA, IHC (p), IF und RIA. Er zeigt eine Reaktivität gegenüber Maus.

Kurzübersicht für Collagen IV Antikörper (ABIN5680248)

Target: Collagen IV (COL4) (Collagen, Type IV (COL4))

Reaktivität: Maus

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Dieser Collagen IV Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), ELISA, Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunofluorescence (IF), Radioimmunoassay (RIA)

Produktdetails anti-Collagen IV Antikörper

Spezifität: Murine collagen type IV 100 % , murine collagen type I, II and III <0.1 %, human collagen type IV and V <0.1 %, murine fibronectin <0.1 %, murine laminin <0.1 % in RIA at 1:5000 dilution

Kreuzreaktivität (Details): Mouse

Produktmerkmale: Type IV Collagen is a non-fibrillary network of different alpha-chains: alpha 1(IV) to-alpha 6(IV). It is typically found in basal membranes of different organs (e.g. skin, lens, lung, kidney). Collagens consist of a family of highly specialized glycoproteins of which at least 16 genetically distinct types are known to date. The basal unit of a collagen molecule consists of a triple-helical structure formed by 3 alpha-chains. Predominant amino acids are glycine, proline and hydroxproline. Regularly also lysines and hydroxylysines occur, which are responsible for cross-linkage and glycosylation of the protein chains. Different composition of alpha-chains and different glycosylation contribute to the high variability of collagens in different tissues and organs. Murine collagen type IV 100 % , murine collagen type I, II and III <0.1 %, human collagen type IV and V <0.1 %, murine fibronectin <0.1 %, murine laminin <0.1 % in RIA at 1:5000 dilution.

Aufreinigung: Affinity purified

Immunogen: Purified collagen type IV from murine tumour tissue

Isotyp: IgG

Anwendungsinformationen

Applikationshinweise: After de-waxing the tissue slices they are treated with 0.2 % hyaluronidase (app. 300 U/mg ) in TBS 15 min at 37 °C. Thereafter non-specific binding is blocked by blocking serum or 3 % BSA in TBS. For peroxidase systems blocking with 1 % peroxide solution in TBS for 30 min at RT is recommended.,Mouse skin or liver,IHC(P), IFA, ELISA, RIA, IB/WB,IHC(P) 60 min at RT or 2-8 °C over night,(purified, lyophilized) IFA ? 1:80, IHC(P) ? 1:500, ELISA ? 1:200 (OD ? 500)

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Lyophilized

Konzentration: 1 mg/mL

Buffer: phosphate buffered solution, no BSA and preservative added!

Konservierungsmittel: Without preservative

Lagerung: -20 °C

Details zu Collagen IV

Target: Collagen IV (COL4) (Collagen, Type IV (COL4))

Andere Bezeichnung: Collagen Type IV