Collagen IV Antikörper

Produktnummer ABIN5680242

Dieses Maus Monoklonal-Antikörper erkennt spezifisch Collagen IV in WB, IHC (fro) und IHC (p). Er zeigt eine Reaktivität gegenüber Human.

Kurzübersicht für Collagen IV Antikörper (ABIN5680242)

Target: Collagen IV (COL4) (Collagen, Type IV (COL4))

Reaktivität: Human

Wirt: Maus

Klonalität: Monoklonal

Konjugat: Dieser Collagen IV Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), Immunohistochemistry (Frozen Sections) (IHC (fro)), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Klon: COL 94

Produktdetails anti-Collagen IV Antikörper

Spezifität: Human Collagen IV, no cross-reactivity with other Collagen types, Vitronectin, Fibronectin or Chondroitinsulfate Human and other primates, no reactivity in chicken

Keine Kreuzreaktivität: Cat, Huhn, Ziege, Kaninchen, Ratte, Schaf

Kreuzreaktivität (Details): Human and other primates (no reactivity in chicken, cat, rabbit, rat, sheep and goat)

Produktmerkmale: Type IV Collagen is a non-fibrillary network of different alpha-chains: alpha 1(IV) to-alpha 6(IV). It is typically found in basal membranes of different organs (e.g. skin, lens, lung, kidney). Collagens consist of a family of highly specialized glycoproteins of which at least 16 genetically distinct types are known to date. The basal unit of a collagen molecule consists of a triple-helical structure formed by 3 alpha-chains. Predominant amino acids are glycine, proline and hydroxproline. Regularly also lysines and hydroxylysines occur, which are responsible for cross-linkage and glycosylation of the protein chains. Different composition of alpha-chains and different glycosylation contribute to the high variability of collagens in different tissues and organs. Human Collagen IV, no cross-reactivity with other Collagen types, Vitronectin, Fibronectin or Chondroitinsulfate.

Aufreinigung: Antibody solution

Immunogen: Purified human Collagen IV

Isotyp: IgG1

Anwendungsinformationen

Applikationshinweise: After de-waxing the tissue slices they are treated with 0.2 % hyaluronidase (app. 300 U/mg ) in TBS 15 min at 37 °C. Thereafter non-specific binding is blocked by blocking serum or 3 % BSA in TBS. For peroxidase systems blocking with 1 % peroxide solution in TBS for 30 min at RT is recommended.,Human skin, placenta or lens capsule,IHC(C,P), WB,60 min at RT,(liquid conc.) 1:10 - 1:50

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Konzentration: 100 μg/mL

Buffer: stabilizing phosphate buffer  pH 7.3. Contains 0.09 % sodium azide. The volume is sufficient for at least 100 immunohistochemical tests (100 μL working solution / test)

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Lagerung: 4 °C

Details zu Collagen IV

Target: Collagen IV (COL4) (Collagen, Type IV (COL4))

Andere Bezeichnung: Collagen Type IV