TNF alpha Antikörper

Produktnummer ABIN5596797

Hersteller Produkt- Nr.: 210-401-321-0100

Der Kaninchen Polyklonal Anti-TNF alpha-Antikörper wurde für WB, ELISA, IHC und IP validiert. Er ist geeignet, TNF alpha in Proben von Maus zu detektieren. Es ist 1 Publikation verfügbar.

Kurzübersicht für TNF alpha Antikörper (ABIN5596797)

Target: TNF alpha (Tumor Necrosis Factor alpha (TNF alpha))

Reaktivität: Maus

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Dieser TNF alpha Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), ELISA, Immunohistochemistry (IHC), Immunoprecipitation (IP)

Produktdetails anti-TNF alpha Antikörper

Hersteller Produkt- Nr.: 210-401-321-0100

Hersteller: Rockland

Verwendungszweck: TNF alpha Antibody

Kreuzreaktivität (Details): The antibody is directed against mature 17,000 MW mouse TNFα and is useful in determining its presence in various assays.

Produktmerkmale: Synonyms: rabbit anti-TNF alpha antibody, rabbit anti-TNFa antibody, rabbit anti-tumor necrosis factor alpha antibody, APC1 antibody, Cachectin antibody, DIF antibody, Differentiation inducing factor antibody, Macrophage cytotoxic factor antibody, MCF antibody, Necrosin antibody, TNF-alpha, Tumor necrosis factor ligand superfamily member 2, TNF-a, TNFA

Aufreinigung: TNF alpha Antibody has been heated to 56°C for 30 minutes.

Sterilität: Sterile filtered

Immunogen:

Immunogen: This antibody was produced from whole rabbit serum prepared by repeated immunizations with recombinant mouse TNFalpha produced in E.coli.

Immunogen Type: Recombinant Protein

Isotyp: IgG

Anwendungsinformationen

Applikationshinweise:

Immunohistochemistry Dilution: User Optimized

Application Note: This antibody against Mouse TNFα has been tested for use in immunoblotting.  This antibody is suitable for ELISA, immunohistochemistry, neutralizations, radioimmunoassay, and immunoprecipitation. It recognizes the 17,000 MW TNFα.  Reactivity in other immunoassays is unknown. This antibody will recognize the cell-bound precursor of TNFα as a 26,000 protein in immunoblots, particularly in denatured samples. This antibody is also useful for neutralization of mouse activity in bioassays. It does not neutralize the biological activity of lymphotoxin. For neutralization, it is recommended to incubate the sample with a 1:200 dilution of the antibody for at least 4 hours before being tested. A control of similarly diluted normal rabbit IgG is recommended.

Neutralization Dilution: User Optimized

Western Blot Dilution: 1:500 - 1:2,000

Immunoprecipitation Dilution: User Optimized

ELISA Dilution: 1:1,000 - 1:5,000

Other: User Optimized

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Konzentration: 1.0 mg/mL

Buffer:

Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2

Stabilizer: None

Preservative: 0.01 % (w/v) Sodium Azide

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Lagerung: 4 °C,-20 °C

Informationen zur Lagerung: Store vial at -20° C prior to opening. Aliquot contents and freeze at -20° C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.

Haltbarkeit: 12 months

Referenzen für anti-TNF alpha Antikörper (ABIN5596797)

Wang, Biswas, Ma, Kandpal, Coker, Grandgenett, Hollingsworth, Jain, Tanji, Lόpez-Pintado, Borczuk, Hebert, Jenkitkasemwong, Hojyo, Davuluri, Knutson, Fukada, Acharyya: "Metastatic cancers promote cachexia through ZIP14 upregulation in skeletal muscle." in: Nature medicine, Vol. 24, Issue 6, pp. 770-781, (2019) (PubMed).

Details zu TNF alpha

Target: TNF alpha (Tumor Necrosis Factor alpha (TNF alpha))

Andere Bezeichnung: Tnf

Hintergrund: Background: TNF alpha Antibody detects TNF-a protein. TNF-a is a cytokine that binds to TNFRSF1A/TNFR1 and TNFRSF1B/TNFBR. It is mainly secreted by macrophages and can induce cell death of certain tumor cell lines. It is a potent pyrogen causing fever by direct action or by stimulation of interleukin-1 secretion and is implicated in the induction of cachexia, Under certain conditions it can stimulate cell proliferation and induce cell differentiation. Anti-TNF-alpha Antibody is ideal for investigators involved in Cell Signaling, Immunology and Signal Transduction research.

Gen-ID: 21926

UniProt: P06804

Pathways: NF-kappaB Signalweg, Apoptose, Caspase Kaskade in der Apoptose, TLR Signalweg, Cellular Response to Molecule of Bacterial Origin, Regulation of Leukocyte Mediated Immunity, Positive Regulation of Immune Effector Process, Production of Molecular Mediator of Immune Response, Positive Regulation of Endopeptidase Activity, Hepatitis C, Protein targeting to Nucleus, Inflammasome