Collagen IV Antikörper

Produktnummer ABIN5541082

Dieses Anti-Collagen IV-Antikörper ist ein Maus Monoklonal-Antikörper zur Detektion von Collagen IV in WB, IHC (fro), IHC (p), FACS, IF und ICC. Geeignet für Human, Schwein und Rind (Kuh).

Kurzübersicht für Collagen IV Antikörper (ABIN5541082)

Target: Collagen IV (COL4) (Collagen, Type IV (COL4))

Reaktivität: Human, Schwein, Rind (Kuh)

Wirt: Maus

Klonalität: Monoklonal

Konjugat: Dieser Collagen IV Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), Immunohistochemistry (Frozen Sections) (IHC (fro)), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Flow Cytometry (FACS), Immunofluorescence (IF), Immunocytochemistry (ICC)

Klon: 1043

Produktdetails anti-Collagen IV Antikörper

Spezifität: This antibody clone 1043 recognizes type IV collagen in the extracellular - basement membranes of normal and pathological tissues.

Kreuzreaktivität (Details): Does NOT react with Rat and Rabbit.

Aufreinigung: Purified

Immunogen: Purified Human type IV Collagen, isolated from Human placenta. Antibody derived by fusion of Sp 2/0 mouse myeloma cells with spleen cells from a BALB/c mouse.

Isotyp: IgG1

Anwendungsinformationen

Applikationshinweise: Western blot : 1/100-1/1000. Immunocytochemistry on fixed cells (methanol fixation). Flow cytometry. Immunohistochemistry on Frozen Sections using a PBS buffer containing 0.1 mM CaCl 2 and 0.1 mM MgCl. Immunohistochemistry on Paraffin Sections : Pretreatement with 1 mg/mL pepsin in 0.01N HCl for 30 minutes at room temperature is recommended. Recommended Dilutions : 1/50-1/100 for Immunohistochemistry with avidin-biotinylated horseradish peroxidase complex (ABC) as detection reagent.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Buffer: Buffer System: PBS Preservatives: 0.09 % Sodium Azide

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Lagerung: 4 °C,-20 °C

Informationen zur Lagerung: Store undiluted at 2-8°C for one month or (in aliquots) at -20°C for longer. Avoid repeated freeze-thaw cycles. Shelf life: One year from despatch.

Details zu Collagen IV

Target: Collagen IV (COL4) (Collagen, Type IV (COL4))

Andere Bezeichnung: collagen type iv

Hintergrund: Type IV collagen is an integral part of most, if not all basement membranes. The extent of expression of the basement membrane component type IV collagen may be correlated with the degree of differentiation of basement membrane producing neoplasms. Neoplastic cells of invasive carcinomas often lack a continuous basement membrane. In diagnostic histopathology antibodies to type IV collagen may help to distinguish between non-invasive (benign or in situ) and invasive lesions. Furthermore the antiserum can be of help in the differential diagnosis of bullous lesions in dermatopathology.

UniProt: P02462