CD11b Antikörper (N-Term)

Produktnummer ABIN5518692

Dieses Kaninchen Polyklonal-Antikörper erkennt spezifisch CD11b in WB. Er zeigt eine Reaktivität gegenüber Human, Maus und Ratte und wurde in 6+ Publikationen erwähnt.

Kurzübersicht für CD11b Antikörper (N-Term) (ABIN5518692)

Target: CD11b (ITGAM) (Integrin alpha M (ITGAM))

Reaktivität: Human, Maus, Ratte

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Dieser CD11b Antikörper ist unkonjugiert

Applikation: Western Blotting (WB)

Produktdetails anti-CD11b Antikörper

Bindungsspezifität: AA 177-192, N-Term

Verwendungszweck: Anti-CD11b/ITGAM Antibody Picoband®

Sequenz: MEQLKKSKTL FSLMQY

Kreuzreaktivität (Details): No cross-reactivity with other proteins

Produktmerkmale: Anti-CD11b/ITGAM Antibody (ABIN5518692). Tested in WB applications. This antibody reacts with Human, Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.

Aufreinigung: Immunogen affinity purified.

Immunogen: A synthetic peptide corresponding to a sequence at the N-terminus of human CD11b, different from the related mouse sequence by one amino acid, and from the related rat sequence by three amino acids.

Isotyp: IgG

Anwendungsinformationen

Applikationshinweise: Western blot, 0.1-0.5 μg/mL, Human, Mouse, Rat
1. Callen, D. F., Chen, L. Z., Nancarrow, J., Whitmore, S. A., Apostolou, S., Thompson, A. D., Lane, S. A., Stallings, R. L., Hildebrand, C. E., Harris, P. G., Sutherland, G. R. Current state of the physical map of human chromosome 16. (Abstract) Cytogenet. Cell Genet. 58: 1998 only, 1991. 2. Corbi, A. L., Larson, R. S., Kishimoto, T. K., Springer, T. A., Morton, C. C. Chromosomal location of the genes encoding the leukocyte adhesion receptors LFA-1, Mac-1 and p150,95: identification of a gene cluster involved in cell adhesion. J. Exp. Med. 167: 1597-1607, 1988. 3. Simon, D. I., Chen, Z., Seifert, P., Edelman, E. R., Ballantyne, C. M., Rogers, C. Decreased neointimal formation in Mac-1 -/- mice reveals a role for inflammation in vascular repair after angioplasty. J. Clin. Invest. 105: 293-300, 2000.

Kommentare:

Antibody can be supported by chemiluminescence kit ABIN921124 in WB.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Lyophilized

Rekonstitution: Add 0.2 mL of distilled water will yield a concentration of 500 μg/mL.

Konzentration: 500 μg/mL

Buffer: Each vial contains 5 mg BSA, 0.9 mg NaCl, 0.2 mg Na2HPO4, 0.05 mg Thimerosal, 0.05 mg Sodium azide.

Konservierungsmittel: Sodium azide, Thimerosal (Merthiolate)

Vorsichtsmaßnahmen: This product contains Thimerosal (Merthiolate) and Sodium azide: POISONOUS AND HAZARDOUS SUBSTANCES which should be handled by trained staff only.

Lagerung: 4 °C,-20 °C

Informationen zur Lagerung: Store at -20°C for one year from date of receipt. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freeze-thaw cycles.

Referenzen für anti-CD11b Antikörper (ABIN5518692)

Mu, Jing, Guo: "Expressions of CD11a, CD11b, and CD11c integrin proteins in rats with myocardial hypertrophy." in: Iranian journal of basic medical sciences, Vol. 17, Issue 11, pp. 874-8, (2015) (PubMed).

Ren, Zhang, Zhou, Sun, Zhang, Wei, Zhang, Si: "Tanshinone IIA prevents the loss of nigrostriatal dopaminergic neurons by inhibiting NADPH oxidase and iNOS in the MPTP model of Parkinson's disease." in: Journal of the neurological sciences, Vol. 348, Issue 1-2, pp. 142-52, (2015) (PubMed).

Zhao, Wang, Wang, Sun, Yang, Qiu, Wu, Zhang, Li, Zhang, Zhao, Zou, Ge: "CXCR6 deficiency ameliorated myocardial ischemia/reperfusion injury by inhibiting infiltration of monocytes and IFN-?-dependent autophagy." in: International journal of cardiology, Vol. 168, Issue 2, pp. 853-62, (2013) (PubMed).

Yuan, Su, Pu, Liu, Chen, Zhu, Zhu, Zhang, He: "Ethyl pyruvate promotes spinal cord repair by ameliorating the glial microenvironment." in: British journal of pharmacology, Vol. 166, Issue 2, pp. 749-63, (2012) (PubMed).

Leng, Zhan, Wang, Liu, Gong, Gao, Wu, Wang, Zhao, Wang, Zhang, Pang, Qian: "Anti-heat shock protein 70 autoantibody epitope changes and BD091 promotes atherosclerosis in rats." in: Cell stress & chaperones, Vol. 15, Issue 6, pp. 947-58, (2011) (PubMed).

Shi, Xun, Yue, Zhang, Ren, Wang, Wu, Shi, Yang, Lei: "Cloning, characterization, and expression analysis of goat (Capra hircus) phospholipid hydroperoxide glutathione peroxidase (PHGPx)." in: International journal of biological sciences, Vol. 6, Issue 4, pp. 316-26, (2010) (PubMed).

Details zu CD11b

Target: CD11b (ITGAM) (Integrin alpha M (ITGAM))

Andere Bezeichnung: ITGAM

Hintergrund:

Synonyms: Integrin alpha-M,CD11 antigen-like family member B,CR-3 alpha chain,Cell surface glycoprotein MAC-1 subunit alpha,Leukocyte adhesion receptor MO1,Neutrophil adherence receptor,CD11b,ITGAM,CD11B, CR3A,

Tissue Specificity: Predominantly expressed in monocytes and granulocytes.

Background: ITGAM (Integrin Alpha-M), also called CD11B or Mo1 ALPHA SUBUNIT (MO1A), is one protein subunit that forms the heterodimeric integrin alpha-M beta-2 (alphaMbeta2) molecule. A major surface antigen family on human leukocytes includes complement receptor type 3 (CR3A, also called ITGAM, Mac1, or Mo1), lymphocyte function-associated (LFA) antigen type 1 (ITGAL), and p150, 95 (ITGAX). By in situ hybridization, Corbi et al. (1988) demonstrated that the genes encoding the alpha subunits of LFA1 (ITGAL), Mac1, and p150, 95 (ITGAX), all of which are involved in leukocyte adhesion, constitute a cluster on 16p13.1-p11. Callen et al. (1991) narrowed the assignment to 16p11.2. Inflammation plays an essential role in the initiation and progression of atherosclerosis. Simon et al. (2000) presented evidence that it also has a role in vascular repair after mechanical arterial injury (i.e., percutaneous transluminal coronary angioplasty, or PTCA).

Sequence Similarities: Belongs to the integrin alpha chain family.

Molekulargewicht: 170 kDa

UniProt: P11215

Pathways: Apoptose, Activation of Innate immune Response, Toll-Like Receptors Cascades, Activated T Cell Proliferation