BAD Antikörper (pSer118) (AbBy Fluor® 750)

Produktnummer ABIN4997154

Dieses Kaninchen Polyklonal-Antikörper erkennt spezifisch BAD in WB, IF (p), IF (cc) und FACS. Er zeigt eine Reaktivität gegenüber Human, Maus und Ratte.

Kurzübersicht für BAD Antikörper (pSer118) (AbBy Fluor® 750) (ABIN4997154)

Target: BAD (BCL2-Associated Agonist of Cell Death (BAD))

Reaktivität: Human, Maus, Ratte

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Dieser BAD Antikörper ist konjugiert mit AbBy Fluor® 750

Applikation: Western Blotting (WB), Immunofluorescence (Paraffin-embedded Sections) (IF (p)), Immunofluorescence (Cultured Cells) (IF (cc)), Flow Cytometry (FACS)

Produktdetails anti-BAD Antikörper (AbBy Fluor® 750)

Bindungsspezifität: pSer118

Spezifität: KO-Validated

Kreuzreaktivität: Human, Maus, Ratte

Homologie: Dog,Cow,Pig,Horse,Rabbit

Aufreinigung: Purified by Protein A.

Immunogen: KLH conjugated synthetic phosphopeptide derived from human BAD around the phosphorylation site of Ser118

Isotyp: IgG

Anwendungsinformationen

Applikationshinweise: FCM 1:20-100
IF(IHC-P) 1:50-200
IF(IHC-F) 1:50-200
IF(ICC) 1:50-200

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Konzentration: 1 μg/μL

Buffer: Aqueous buffered solution containing 0.01M TBS ( pH 7.4) with 1 % BSA, 0.03 % Proclin300 and 50 % Glycerol.

Konservierungsmittel: ProClin

Vorsichtsmaßnahmen: This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE, which should be handled by trained staff only.

Lagerung: -20 °C

Informationen zur Lagerung: Store at -20°C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles.

Haltbarkeit: 12 months

Details zu BAD

Target: BAD (BCL2-Associated Agonist of Cell Death (BAD))

Andere Bezeichnung: Bad

Hintergrund:

Synonyms: Bad phospho S118, Bad phospho Ser118, p-Bad S118,p- Bad Ser118, p-Bad phospho Ser118, BBC 2, BBC2, BBC6, Bcl 2 Antagonist of Cell Death, Bcl 2 Binding Component 6, BCL X / BCL 2 Binding Protein, mouse BAD Ser155, rat BAD Ser156

Background: Bad is a member of the Bcl2 family and acts to promote apoptosis by forming heterodimers with the survival proteins Bcl2 and BclxL, thus preventing them from binding with BAX. Bad is found on the outer mitochondrial membrane and, once phosphorylated in response to growth stimuli, translocates to the cytoplasm. The phosphorylation status of Bad represents a key checkpoint for death or cell survival. JNK-induced phosphorylation of BAD serine 128 promotes the apoptotic role of Bad by opposing the inhibitory effect of growth factor on Bad-mediated apoptosis. Cdc2-induced phosphorylation of Bad serine 128 has an inhibitory effect on its interaction with 14-3-3 proteins. The latter interaction is critical for Bad phosphorylation at serine 155, a site within the BH3 domain that leads to the release of BclxL and the promotion of cell survival. Alternative splicing of this gene results in two transcript variants which encode the same isoform.

Gen-ID: 572

UniProt: Q92934

Pathways: MAPK Signalweg, PI3K-Akt Signalweg, RTK Signalweg, Apoptose, Fc-epsilon Rezeptor Signalübertragung, Positive Regulation of Peptide Hormone Secretion, Carbohydrate Homeostasis, Positive Regulation of Endopeptidase Activity, Regulation of Carbohydrate Metabolic Process, Hepatitis C, CXCR4-mediated Signaling Events