Ephrin B2 Antikörper (Internal Region)

Produktnummer ABIN499146

Dieses Anti-Ephrin B2-Antikörper ist ein Ziege Polyklonal-Antikörper zur Detektion von Ephrin B2 in WB und EIA. Geeignet für Human, Maus, Ratte, Rind (Kuh), Hund und Schwein.

Kurzübersicht für Ephrin B2 Antikörper (Internal Region) (ABIN499146)

Target: Ephrin B2 (EFNB2)

Reaktivität: Human, Maus, Ratte, Rind (Kuh), Hund, Schwein

Wirt: Ziege

Klonalität: Polyklonal

Konjugat: Dieser Ephrin B2 Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), Enzyme Immunoassay (EIA)

Produktdetails anti-Ephrin B2 Antikörper

Bindungsspezifität: Internal Region

Sequenz: CPKVDSKTVG QYE

Spezifität: Recognizes EFNB2

Kreuzreaktivität (Details): Species reactivity (expected):Canine, Bovine, Pig.
Species reactivity (tested):Human, Mouse, Rat.

Aufreinigung: Ammonium Sulphate Precipitation followed by Antigen Affinity Chromatography using the immunizing peptide.

Immunogen: Peptide with sequence from the internal region of the protein sequence according to NP_004084.1. Genename: EFNB2

Anwendungsinformationen

Applikationshinweise: Peptide ELISA: Limit Dilution: 1/16000. Western blot: 0.1-0.3 μg/mL. This antibody detects a band of approx. 35 kDa band observed inHuman, Mouse and Rat Lung lysates.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Konzentration: 0.5 mg/mL

Buffer: Tris saline, pH 7.3 containing 0.02 % Sodium Azide as preservative and 0.5 % BSA as stabilizer.

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handhabung: Avoid repeated freezing and thawing.

Lagerung: 4 °C/-20 °C

Informationen zur Lagerung: Store undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer.

Details zu Ephrin B2

Target: Ephrin B2 (EFNB2)

Andere Bezeichnung: Ephrin-B2

Hintergrund: Ephrin receptors and their ligands, the ephrins, mediate numerous developmental processes, particularly in the nervous system. Based on their structures and sequence relationships, ephrins are divided into the ephrin-A (EFNA) class, which are anchored to the membrane by a glycosylphosphatidylinositol linkage, and the ephrin-B (EFNB) class, which are transmembrane proteins. The Eph family of receptors are divided into 2 groups based on the similarity of their extracellular domain sequences and their affinities for binding ephrin-A and ephrin-B ligands. Ephrin receptors make up the largest subgroup of the receptor tyrosine kinase (RTK) family. The ligand-activated form of EphB2, which belongs to the Tyr family of protein kinases, interacts with multiple proteins, including GTPase-activating protein (RASGAP)and also interacts with PRKCABP and GRIP1. This type I membrane protein is expressed in brain, heart, lung, kidney, placenta, pancreas, liver and skeletal muscle. It is preferentially expressed in fetal brain. Ephrin-B2 also binds EphA4, EphB1, EphB2, EphB3 and EphB4. Ephrin-B2 can be detected on monocytes, mesangial cells, CD34+ stem cells, bone marrow fibroblasts, activated melanocytes and melanoma cells, and pre-artery endothelial cells.Synonyms: EFNB2, EPH-related receptor tyrosine kinase ligand 5, EPLG5, HTK ligand, HTK-L, HTKL, LERK-5, LERK5

Molekulargewicht: 36.9kDa (NP_004084.1)

Gen-ID: 1948

NCBI Accession: NP_004084

UniProt: P52799

Pathways: RTK Signalweg, Regulation of Muscle Cell Differentiation