Caspase 9 Antikörper
-
- Target Alle Caspase 9 (CASP9) Antikörper anzeigen
- Caspase 9 (CASP9) (Caspase 9, Apoptosis-Related Cysteine Peptidase (CASP9))
-
Reaktivität
- Human
-
Wirt
- Kaninchen
-
Klonalität
- Polyklonal
-
Konjugat
- Dieser Caspase 9 Antikörper ist unkonjugiert
-
Applikation
- Western Blotting (WB), Immunofluorescence (IF), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))
- Spezifität
- This antibody detects endogenous levels of Caspase 9 protein.
- Kreuzreaktivität (Details)
- Species reactivity (tested):Human.
- Aufreinigung
- Affinity chromatography using epitope-specific immunogen
- Reinheit
- > 95 % (by SDS-PAGE)
- Top Product
- Discover our top product CASP9 Primärantikörper
-
-
- Applikationshinweise
-
Western blot: 1: 500 - 1: 1000. Immunohistochemistry on paraffin sections: 1: 50 - 1: 200. Immunoflourescence: 1: 50 - 1: 200.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user. - Beschränkungen
- Nur für Forschungszwecke einsetzbar
-
- Konzentration
- 1.0 mg/mL
- Buffer
- Phosphate buffered saline (PBS) with 0.05 % sodium azide, approx. pH 7.2
- Konservierungsmittel
- Sodium azide
- Vorsichtsmaßnahmen
- This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- Handhabung
- Avoid repeated freezing and thawing.
- Lagerung
- 4 °C/-20 °C
- Informationen zur Lagerung
- Store at 2 - 8 °C for up to one month or (in aliquots) at -20 °C for longer.
-
- Target
- Caspase 9 (CASP9) (Caspase 9, Apoptosis-Related Cysteine Peptidase (CASP9))
- Andere Bezeichnung
- Caspase-9 (CASP9 Produkte)
- Synonyme
- CASP9 antikoerper, AI115399 antikoerper, APAF-3 antikoerper, AW493809 antikoerper, CASP-9 antikoerper, Caspase-9 antikoerper, ICE-LAP6 antikoerper, Mch6 antikoerper, Apaf3 antikoerper, Casp-9-CTD antikoerper, Casp9_v1 antikoerper, Ice-Lap6 antikoerper, APAF3 antikoerper, MCH6 antikoerper, PPP1R56 antikoerper, caspase 9 antikoerper, CASP9 antikoerper, Casp9 antikoerper
- Hintergrund
- Caspase 9 (also known as ICE like apoptotic protease 6 (ICE LAP6), apoptotic protease Mch6, and apoptotic protease activating factor 3 (Apaf3)) is a member of the peptidase family C14 that contains a CARD domain. This caspase is active as a heterotetramer and has been reported to have two isoforms. Pr °Caspase 9 has been reported to be approximately 47 kD. This caspase is present in the cytosol and, upon activation, translocates to the mitochondria. Caspase 9 is involved in the caspase activation cascade responsible for apoptosis execution and cleaves/activates Caspase 3 and Caspase 6. Caspase 9 is inhibited by the dominant negative isoform, BclXL, cIAP1, cIAP2, XIAP, and Livin. This caspase becomes activated when recruited to Apaf1/cytochrome c complex, and following cleavage by Apaf1, granzyme B, Caspase 3, possibly Caspase 8 and Caspase 10 into large p37 and small p10 subunits. Caspase 9 intereacts with BIRC7 and has been shown to cleave PARP and vimentin.Synonyms: APAF-3, APAF3, Apoptotic protease Mch-6, Apoptotic protease-activating factor 3, CASP-9, CASP9, ICE-LAP6, ICE-like apoptotic protease 6, MCH6
- Molekulargewicht
- approx. 35 kDa
- Gen-ID
- 842
- NCBI Accession
- NP_001220
- UniProt
- P55211
- Pathways
- MAPK Signalweg, RTK Signalweg, Apoptose, Caspase Kaskade in der Apoptose, Fc-epsilon Rezeptor Signalübertragung, EGFR Signaling Pathway, Neurotrophin Signalübertragung, Positive Regulation of Endopeptidase Activity
-