p21 Antikörper (pThr57)

Produktnummer ABIN3031126

Dieser Kaninchen Polyklonal Antikörper detektiert spezifisch p21 in IHC, ELISA und DB. Es zeigt Reaktivität gegenüber Proben von Human.

Kurzübersicht für p21 Antikörper (pThr57) (ABIN3031126)

Target: p21 (CDKN1A) (Cyclin-Dependent Kinase Inhibitor 1A (p21, Cip1) (CDKN1A))

Reaktivität: Human

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Dieser p21 Antikörper ist unkonjugiert

Applikation: Immunohistochemistry (IHC), ELISA, Dot Blot (DB)

Produktdetails anti-p21 Antikörper

Bindungsspezifität: pThr57

Verwendungszweck: p21 Antibody / CDKN1A (pT57)

Aufreinigung: Antigen affinity purified

Immunogen: This phosphorylated-p21 antibody was produced from rabbits immunized with a KLH conjugated synthetic phosphopeptide corresponding to amino acid residues surrounding pT57 of human P21CIP1.

Isotyp: Ig Fraction

Anwendungsinformationen

Applikationshinweise: Titration of the phosphorylated-p21 antibody may be required due to differences in protocols and secondary/substrate sensitivity.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Buffer: In 1X PBS, pH 7.4, with 0.09 % sodium azide

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handhabung: Avoid repeated freeze-thaw cycles

Lagerung: -20 °C

Informationen zur Lagerung: Aliquot the phosphorylated-p21 antibody and store frozen at -20oC or colder. Avoid repeated freeze-thaw cycles.

Details zu p21

Target: p21 (CDKN1A) (Cyclin-Dependent Kinase Inhibitor 1A (p21, Cip1) (CDKN1A))

Andere Bezeichnung: p21

Hintergrund: This gene encodes a potent cyclin-dependent kinase inhibitor. The encoded protein binds to and inhibits the activity of cyclin-CDK2 or -CDK4 complexes, and thus functions as a regulator of cell cycle progression at G1. The expression of this gene is tightly controlled by the tumor suppressor protein p53, through which this protein mediates the p53-dependent cell cycle G1 phase arrest in response to a variety of stress stimuli. This protein can interact with proliferating cell nuclear antigen (PCNA), a DNA polymerase accessory factor, and plays a regulatory role in S phase DNA replication and DNA damage repair. This protein was reported to be specifically cleaved by CASP3-like caspases, which thus leads to a dramatic activation of CDK2, and may be instrumental in the execution of apoptosis following caspase activation. Two alternatively spliced variants, which encode an identical protein, have been reported.

UniProt: P38936

Pathways: p53 Signalweg, PI3K-Akt Signalweg, Zellzyklus, AMPK Signaling, Fc-epsilon Rezeptor Signalübertragung, EGFR Signaling Pathway, Neurotrophin Signalübertragung, Mitotic G1-G1/S Phases, DNA Replication, Hepatitis C, Synthesis of DNA, Autophagie