p21 Antikörper (pThr57)

Produktnummer ABIN3031126

Dieses Kaninchen Polyklonal-Antikörper erkennt spezifisch p21 in IHC, ELISA und DB. Er zeigt eine Reaktivität gegenüber Human.

Kurzübersicht für p21 Antikörper (pThr57) (ABIN3031126)

Target: p21 (CDKN1A) (Cyclin-Dependent Kinase Inhibitor 1A (p21, Cip1) (CDKN1A))

Reaktivität: Human

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Dieser p21 Antikörper ist unkonjugiert

Applikation: Immunohistochemistry (IHC), ELISA, Dot Blot (DB)

Produktdetails anti-p21 Antikörper

Bindungsspezifität: pThr57

Aufreinigung: Antigen affinity purified

Immunogen: This phosphorylated-p21 antibody was produced from rabbits immunized with a KLH conjugated synthetic phosphopeptide corresponding to amino acid residues surrounding pT57 of human P21CIP1.

Isotyp: Ig Fraction

Anwendungsinformationen

Applikationshinweise: Titration of the phosphorylated-p21 antibody may be required due to differences in protocols and secondary/substrate sensitivity.\. Dot blot: 1:500,IHC (Paraffin): 1:50-1:100

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Buffer: In 1X PBS pH 7.4 with 0.09 % sodium azide

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Lagerung: -20 °C

Informationen zur Lagerung: Aliquot the phosphorylated-p21 antibody and store frozen at -20°C or colder. Avoid repeated freeze-thaw cycles.

Details zu p21

Target: p21 (CDKN1A) (Cyclin-Dependent Kinase Inhibitor 1A (p21, Cip1) (CDKN1A))

Andere Bezeichnung: p21

Hintergrund: This gene encodes a potent cyclin-dependent kinase inhibitor. The encoded protein binds to and inhibits the activity of cyclin-CDK2 or -CDK4 complexes, and thus functions as a regulator of cell cycle progression at G1. The expression of this gene is tightly controlled by the tumor suppressor protein p53, through which this protein mediates the p53-dependent cell cycle G1 phase arrest in response to a variety of stress stimuli. This protein can interact with proliferating cell nuclear antigen (PCNA), a DNA polymerase accessory factor, and plays a regulatory role in S phase DNA replication and DNA damage repair. This protein was reported to be specifically cleaved by CASP3-like caspases, which thus leads to a dramatic activation of CDK2, and may be instrumental in the execution of apoptosis following caspase activation. Two alternatively spliced variants, which encode an identical protein, have been reported.

UniProt: P38936

Pathways: p53 Signalweg, PI3K-Akt Signalweg, Zellzyklus, AMPK Signaling, Fc-epsilon Rezeptor Signalübertragung, EGFR Signaling Pathway, Neurotrophin Signalübertragung, Mitotic G1-G1/S Phases, DNA Replication, Hepatitis C, Synthesis of DNA, Autophagie