AIF Antikörper

Produktnummer ABIN4953229

Der Maus Monoklonal Anti-AIF-Antikörper wurde für WB, IF und ICC validiert. Er ist geeignet, AIF in Proben von Human zu detektieren.

Kurzübersicht für AIF Antikörper (ABIN4953229)

Target: AIF (AIFM1) (Apoptosis-Inducing Factor, Mitochondrion-Associated, 1 (AIFM1))

Reaktivität: Human

Wirt: Maus

Klonalität: Monoklonal

Konjugat: Dieser AIF Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), Immunofluorescence (IF), Immunocytochemistry (ICC)

Klon: 8H1-B10-A12

Produktdetails anti-AIF Antikörper

Aufreinigung: Protein G affinity

Immunogen: A human recombinant partial protein was used as the immunogen for this AIF antibody.

Isotyp: IgG2a

Anwendungsinformationen

Applikationshinweise: The stated application concentrations are suggested starting points. Titration of the AIF antibody may be required due to differences in protocols and secondary/substrate sensitivity.\. Western blot: 1:1000,ICC/IF: 1:200

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Buffer: Antibody in PBS with 50 % Glycerol and 0.03 % ProClin 300

Lagerung: -20 °C

Informationen zur Lagerung: Store the AIF antibody at -20°C.

Details zu AIF

Target: AIF (AIFM1) (Apoptosis-Inducing Factor, Mitochondrion-Associated, 1 (AIFM1))

Andere Bezeichnung: AIFM1 / AIF / Apoptosis-Inducing Factor

Hintergrund: Functions both as NADH oxidoreductase and as regulator of apoptosis. In response to apoptotic stimuli, it is released from the mitochondrion intermembrane space into the cytosol and to the nucleus, where it functions as a proapoptotic factor in a caspase-independent pathway. In contrast, functions as an antiapoptotic factor in normal mitochondria via its NADH oxidoreductase activity. The soluble form (AIFsol) found in the nucleus induces 'parthanatos' i.e. caspase-independent fragmentation of chromosomal DNA. Interacts with EIF3G,and thereby inhibits the EIF3 machinery and protein synthesis, and activates casapse-7 to amplify apoptosis. Plays a critical role in caspase-independent, pyknotic cell death in hydrogen peroxide-exposed cells. Binds to DNA in a sequence-independent manner. [UniProt]

UniProt: O95831

Pathways: Apoptose, Positive Regulation of Endopeptidase Activity, Cell RedoxHomeostasis, Smooth Muscle Cell Migration, Warburg Effekt