Rekombinanter TNF alpha Antikörper

Produktnummer ABIN4949950

Der Kaninchen Monoklonal anti-TNF alpha Antikörper wird verwendet zum Nachweis von TNF alpha in Proben von Human. Er wurde validiert für IF und IHC (p).

Kurzübersicht für Rekombinanter TNF alpha Antikörper (ABIN4949950)

Target: TNF alpha (Tumor Necrosis Factor alpha (TNF alpha))

Antikörpertyp: Recombinant Antibody

Reaktivität: Human

Wirt: Kaninchen

Klonalität: Monoklonal

Konjugat: Dieser TNF alpha Antikörper ist unkonjugiert

Applikation: Immunofluorescence (IF), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Klon: RMTNFA-1

Produktdetails anti-TNF alpha Antikörper

Aufreinigung: Protein A affinity chromatography

Immunogen: Recombinant human protein was used as the immunogen for the TNF-alpha antibody.

Isotyp: IgG kappa

Anwendungsinformationen

Applikationshinweise: Optimal dilution of the TNF-alpha antibody should be determined by the researcher.

1. Staining of formalin-fixed tissues requires boiling tissue sections in 10  mM Citrate buffer,  pH 6.0, for 10-20 min followed by cooling at RT for 20 min.\. Immunofluorescence: 5-10 μg/mL,Immunohistochemistry (FFPE): 5-10 μg/mL for 30 min at RT (1)

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Konzentration: 1 mg/mL

Buffer: 1 mg/mL in 1X PBS, BSA free, sodium azide free

Konservierungsmittel: Azide free

Lagerung: 4 °C,-20 °C

Informationen zur Lagerung: Store the TNF-alpha antibody at 2-8°C (with azide) or aliquot and store at -20°C or colder (without azide).

Details zu TNF alpha

Target: TNF alpha (Tumor Necrosis Factor alpha (TNF alpha))

Andere Bezeichnung: TNF-alpha

Hintergrund: Tumor necrosis factor alpha is a cell signaling protein (cytokine) involved in systemic inflammation and is one of the cytokines that make up the acute phase reaction. It is produced chiefly by activated macrophages, although it can be produced by many other cell types such as CD4+ lymphocytes, NK cells, neutrophils, mast cells, eosinophils, and neurons.

The primary role of TNF is in the regulation of immune cells. TNFa, being an endogenous pyrogen, is able to induce fever, apoptotic cell death, cachexia, inflammation and to inhibit tumorigenesis and viral replication and respond to sepsis via IL1 & IL6 producing cells. Dysregulation of TNF production has been implicated in a variety of human diseases including Alzheimer's disease, cancer, major depression, Psoriasis and inflammatory bowel disease (IBD). [Wiki]

Pathways: NF-kappaB Signalweg, Apoptose, Caspase Kaskade in der Apoptose, TLR Signalweg, Cellular Response to Molecule of Bacterial Origin, Regulation of Leukocyte Mediated Immunity, Positive Regulation of Immune Effector Process, Production of Molecular Mediator of Immune Response, Positive Regulation of Endopeptidase Activity, Hepatitis C, Protein targeting to Nucleus, Inflammasome