GSK3 alpha Antikörper (pTyr215, pTyr278)

Produktnummer ABIN492620

Der Maus Monoklonal anti-GSK3 alpha Antikörper wird verwendet zum Nachweis von GSK3 alpha in Proben von Human und Maus. Er wurde validiert für WB.

Kurzübersicht für GSK3 alpha Antikörper (pTyr215, pTyr278) (ABIN492620)

Target: GSK3 alpha (GSK3a) (Glycogen Synthase Kinase 3 alpha (GSK3a))

Reaktivität: Human, Maus

Wirt: Maus

Klonalität: Monoklonal

Konjugat: Dieser GSK3 alpha Antikörper ist unkonjugiert

Applikation: Western Blotting (WB)

Klon: 6D3

Produktdetails anti-GSK3 alpha Antikörper

Bindungsspezifität: pTyr215, pTyr278

Spezifität: This antibody specifically recognizes GSK3beta phosphorylated at Tyr215 at 46 kDa and GSK3alpha phosphorylated at Tyr278 at 54 kDa in Western blot applications.

Produktmerkmale: Synonyms: Glycogen synthase kinase-3 alpha, GSK3A, GSK-3 alpha, Factor A

Aufreinigung: Subsequent Ultrafiltration and Size Exclusion Chromatography.

Bestandteile: incl. positive Control

Immunogen: Phosphopeptide conjugated to hemocyaninEpitope: Phosphotyrosine 215, Phosphotyrosine 278AA Sequence: N V S pY I C S

Isotyp: IgG1

Anwendungsinformationen

Applikationshinweise: Immunoblotting: 0.5 μg/mL for HRPO/ECL detection. Recommended blocking buffer: Casein/Tween 20 based blocking and blot incubationbuffer AS00002BU-N or AS00002BU-L. Included Positive Control: Cell lysate from pervanadate treated HepG2 cells (See Protocolsfor more details).
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Protokoll: Positive Control Provided. Cell lysate from pervanadate-treated HepG2 cellsDescription: Cell lysate from pervanadate-treated HepG2 cells, hepatocellular carcinoma(Human)Format: Lyophilized cell lysate from from HepG2 cells. Serum starved cells were treated for 15min with pervanadate. Reconstitution: Restore by addition of 200 µl H20. After complete solubilization add 200 µl2x SDS-PAGE sample buffer, mix and incubate at 90°C for 5 min. Storage: Aliquote and store frozen. Avoid repeated freeze/thaw cycles.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Rekonstitution: Restore with 1 mL H2O (15 min, RT).

Buffer: 1 mLPBS with 0.09 % Sodium Azide/PEG and Sucrose

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Lagerung: -20 °C/-80 °C

Informationen zur Lagerung: Store lyophilized (preferably in a desiccator) at -20 °C and reconstituted (aliquote andfreeze in liquid nitrogen) at -80 °C. Avoid repeated freezing and thawing. Thaw aliquots at 37 °C. Thawed aliquots may be stored at 4 °C up to 3 months.
Shelf life: one year from despatch.

Haltbarkeit: 12 months

Details zu GSK3 alpha

Target: GSK3 alpha (GSK3a) (Glycogen Synthase Kinase 3 alpha (GSK3a))

Andere Bezeichnung: GSK3 alpha

Hintergrund: Glycogen Synthase Kinase 3 beta (GSK3beta) is a serine/threonine kinase that is acting independently of most signal transduction pathways. The activity of GSK3beta can be modulated by phosphorylation of Ser9 and Tyr215 in the activation loop. GSK3beta plays a crucial role in phosphorylation of beta-catenin and thus is an important kinase involved in the regulation of beta-catenin/wnt-signaling.Synonyms: Factor A, GSK-3 alpha, GSK3A, Glycogen synthase kinase-3 alpha

Gen-ID: 2931

UniProt: P49840

Pathways: PI3K-Akt Signalweg, WNT Signalweg, Fc-epsilon Rezeptor Signalübertragung, EGFR Signaling Pathway, Neurotrophin Signalübertragung, cAMP Metabolic Process, Cellular Glucan Metabolic Process, Regulation of Muscle Cell Differentiation, Regulation of G-Protein Coupled Receptor Protein Signaling, ER-Nucleus Signaling, Regulation of Carbohydrate Metabolic Process, BCR Signaling, Warburg Effekt