NF-kB p65 Antikörper (C-Term)

Produktnummer ABIN4889656

Es sind 3+ Publikationen für dieses Produkt verfügbar. Der Kaninchen Polyklonal anti-NF-kB p65 Antikörper wird verwendet zum Nachweis von NF-kB p65 in Proben von Human. Er wurde validiert für WB, ChIP, ChIP-seq und SSA.

Kurzübersicht für NF-kB p65 Antikörper (C-Term) (ABIN4889656)

Target: NF-kB p65 (NFkBP65) (Nuclear Factor-kB p65 (NFkBP65))

Reaktivität: Human

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Dieser NF-kB p65 Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), Chromatin Immunoprecipitation (ChIP), ChIP DNA-Sequencing (ChIP-seq), Supershift Assay (SSA)

Produktdetails anti-NF-kB p65 Antikörper

Bindungsspezifität: C-Term

Verwendungszweck: NFkB p65 antibody (pAb)

Aufreinigung: Unpurified

Immunogen: This NFkB p65 antibody was raised against a peptide immunogen corresponding to the C-terminal of human p65.

Anwendungsinformationen

Applikationshinweise: ChIP-Seq: 10 µL per ChIP ChIP: 4 µL per ChIP WB: 1:2,500 - 1:5,000 dilution

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Buffer: Rabbit serum containing 30 % glycerol and 0.035 % sodium azide.

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handhabung: Avoid repeated freeze/thaw cycles by aliquoting items into single-use fractions,Keep all reagents on ice when not in storage

Lagerung: -20 °C

Informationen zur Lagerung: Some products may be shipped at room temperature. This will not affect their stability or performance. Avoid repeated freeze/thaw cycles by aliquoting items into single-use fractions for storage at -20°C for up to 2 years. Keep all reagents on ice when not in storage.

Haltbarkeit: 24 months

Referenzen für anti-NF-kB p65 Antikörper (ABIN4889656)

Diermeier, Kolovos, Heizinger, Schwartz, Georgomanolis, Zirkel, Wedemann, Grosveld, Knoch, Merkl, Cook, Längst, Papantonis: "TNF? signalling primes chromatin for NF-?B binding and induces rapid and widespread nucleosome repositioning." in: Genome biology, Vol. 15, Issue 12, pp. 536, (2015) (PubMed).

Raaz, Schellinger, Chernogubova, Warnecke, Kayama, Penov, Hennigs, Salomons, Eken, Emrich, Zheng, Adam, Jagger, Nakagami, Toh, Toyama, Deng, Buerke, Maegdefessel, Hasenfuß, Spin, Tsao: "Transcription Factor Runx2 Promotes Aortic Fibrosis and Stiffness in Type 2 Diabetes Mellitus." in: Circulation research, Vol. 117, Issue 6, pp. 513-24, (2015) (PubMed).

Anderson, Rahmutula, Gardner: "Tumor necrosis factor-alpha inhibits endothelial nitric-oxide synthase gene promoter activity in bovine aortic endothelial cells." in: The Journal of biological chemistry, Vol. 279, Issue 2, pp. 963-9, (2004) (PubMed).

Details zu NF-kB p65

Target: NF-kB p65 (NFkBP65) (Nuclear Factor-kB p65 (NFkBP65))

Andere Bezeichnung: NFkappaB p65

Hintergrund: NFkB p65 (Nuclear factor NF-kappa-B p65 subunit also known as NFkB p65 or RelA) is a member of the Rel family of transcription factors that are involved in processes such as inflammation, immunity, differentiation, cell growth, tumorigenesis and apoptosis. The Rel family includes c-Rel, RelA (NFkB p65, NFkB3) and RelB (I-Rel). Rel proteins form dimers with the NFkB p50 (proteolytically processed from p105/NFkB1) and p52 (proteolytically processed from p100/NFkB2) members of the NFkB family that bind DNA and activate transcription. The NFkB p65/p50 heterodimer is the most abundant form of NFkB. NFkB signaling is negatively regulated by the sequestration of the NFkB complex in the cytoplasm by its association with the IkB family of inhibitory proteins. The IkB Kinase (IKK) complex is the key enzyme involved in the activation and translocation of NFkB. The IKK complex is composed of two catalytic subunits (IKKa and IKKb) and a regulatory subunit (IKKg). Upon its activation, the IKK complex phosphorylates IkB proteins (IkBa, IkBa phospho Ser32, 36), which marks them for ubiquitination and degradation. This enables the NFkB complex to translocate to the nucleus where it regulates transcription by binding to DNA.

Molekulargewicht: 65 kDa

Gen-ID: 5970

NCBI Accession: NP_068810

Pathways: NF-kappaB Signalweg, RTK Signalweg, T-Zell Rezeptor Signalweg, TLR Signalweg, Fc-epsilon Rezeptor Signalübertragung, Neurotrophin Signalübertragung, Activation of Innate immune Response, Cellular Response to Molecule of Bacterial Origin, Hepatitis C, Toll-Like Receptors Cascades, S100 Proteine