LY9 Antikörper (FITC)

Produktnummer ABIN487456

Dieses Maus Monoklonal-Antikörper erkennt spezifisch LY9 in FACS. Er zeigt eine Reaktivität gegenüber Human.

Kurzübersicht für LY9 Antikörper (FITC) (ABIN487456)

Target: LY9 (CD229) (Lymphocyte Antigen 9 (CD229))

Reaktivität: Human

Wirt: Maus

Klonalität: Monoklonal

Konjugat: Dieser LY9 Antikörper ist konjugiert mit FITC

Applikation: Flow Cytometry (FACS)

Klon: HLy9-1-25

Produktdetails anti-LY9 Antikörper (FITC)

Spezifität: This antibody reacts with Human Ly-9 antigen.

Kreuzreaktivität (Details): Species reactivity (tested):Human.

Produktmerkmale: Synonyms: Ly-9, T-lymphocyte surface antigen Ly-9, Lymphocyte antigen 9

Aufreinigung: Protein-A Sepharose Chromatography.

Immunogen: Mouse pre-B-cell line 300. 19, stably transfected with the full length Human Ly-9 cDNA. Remarks: Hybridoma was established by fusion of mouse myeloma cell NS-1 with Balb/cmouse splenocyte.

Isotyp: IgG1

Anwendungsinformationen

Applikationshinweise: Flow Cytometry: 10 μg/mL (final concentration). Positive Control: Peripheral blood T cells.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Protokoll: Flow Cytometric Analysis for Floating CellsProtocol 1We use Fisher tubes or equivalents as reaction tubes for all step described below. 1) Wash the cells 3 times with washing buffer [PBS containing 2% fetal calf serum (FCS) and0. 1% BaN3]2) Add 10 µL of normal goat serum to the cell pellet after tapping. Mix well, and incubatefor 5 minutes at room temperature (20~25°C). 3) Add 30 µL of the FITC labeled CD229 monoclonal antibody (10-20 µg/mL) diluted withPBS containing 2% FCS. Mix well, and incubate for 30 minutes at room temperature(20~25°C). 4) Add 1 mL of the washing buffer followed by centrifugation at 500xg for 1 minute at RT(20~25°C). Remove supernatant by careful aspiration. 5) Resuspend the cells with 500 µL of the washing buffer and analyze by a flow cytomete.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Konzentration: 0.05 mg/mL

Buffer: PBS, 7.2 containing 1 % BSA as stabilizer and 0.09 % Sodium Azide as preservative.

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Lagerung: 4 °C

Informationen zur Lagerung: Store the antibody undiluted at 2-8 °C.
Shelf life: one year from despatch.

Haltbarkeit: 12 months

Details zu LY9

Target: LY9 (CD229) (Lymphocyte Antigen 9 (CD229))

Andere Bezeichnung: CD229 / LY9

Hintergrund: CD229 is a 100-120 kD cell surface glycoprotein that is the homologue of murine Ly-9. CD229 is a member of the immunoglobulin superfamily and may have a function in cell signalling. The antigen is expressed at low levels by mature T and B lymphocytes and at higher levels by single-positive thymocytes. Expression at variable levels is also seen in some cell lines, such as Daudi, U937 and HUT78.Synonyms: Ly-9, Lymphocyte antigen 9, T-lymphocyte surface antigen Ly-9

Gen-ID: 4063

UniProt: Q9HBG7

Pathways: BCR Signaling