LY9 Antikörper (Lymphocyte Antigen 9) (PE) Primary Antibody
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- Target Alle LY9 (CD229) Antikörper anzeigen
- LY9 (CD229)
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Reaktivität
- Human
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Wirt
- Maus
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Klonalität
- Monoklonal
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Konjugat
- Dieser LY9 Antikörper ist konjugiert mit PE
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Applikation
- Functional Studies (Func), Immunocytochemistry (ICC), Flow Cytometry (FACS), Immunoprecipitation (IP), Western Blotting (WB)
- Spezifität
- The mouse monoclonal antibody HLy9.25 (also known as HLy9.1.25) recognizes CD229 / Ly9, a 100-120 kDa cell surface glycoprotein expressed on T and B cells.
- Kreuzreaktivität (Details)
- Human
- Immunogen
- CD299-transfected 300.19 pre-B cell line
- Klon
- HLy9-25
- Isotyp
- IgG1
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- Applikationshinweise
- The reagent is designed for Flow Cytometry analysis of human blood cells using 20 μ,l reagent / 100 μ,l of whole blood or 106 cells in a suspension.The content of a vial (2 ml) is sufficient for 100 tests.
- Kommentare
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The purified antibody is conjugated with R-Phycoerythrin (PE) under optimum conditions. The conjugate is purified by size-exclusion chromatography and adjusted for direct use. No reconstitution is necessary.
- Beschränkungen
- Nur für Forschungszwecke einsetzbar
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- Rekonstitution
- No reconstitution is necessary.
- Buffer
- The reagent is provided in stabilizing phosphate buffered saline (PBS) solution containing 15 mM sodium azide.
- Vorsichtsmaßnahmen
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- Handhabung
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Do not freeze.
Avoid prolonged exposure to light. - Lagerung
- 4 °C
- Informationen zur Lagerung
- Store in the dark at 2-8°C. Do not freeze. Avoid prolonged exposure to light. Do not use after expiration date stamped on vial label.
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CD229 (Ly9) lymphocyte cell surface receptor interacts homophilically through its N-terminal domain and relocalizes to the immunological synapse." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 174, Issue 11, pp. 7033-42, (2005) (PubMed).
: "Molecular characterization and expression of a novel human leukocyte cell-surface marker homologous to mouse Ly-9." in: Blood, Vol. 97, Issue 11, pp. 3513-20, (2001) (PubMed).
: "
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CD229 (Ly9) lymphocyte cell surface receptor interacts homophilically through its N-terminal domain and relocalizes to the immunological synapse." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 174, Issue 11, pp. 7033-42, (2005) (PubMed).
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- Target
- LY9 (CD229)
- Andere Bezeichnung
- CD229 (CD229 Produkte)
- Synonyme
- LY9, CD229, SLAMF3, hly9, mLY9, AI893573, Lgp100, Ly-9, T100, lymphocyte antigen 9, LY9, Ly9
- Hintergrund
- CD229 (Ly9) is a cell surface receptor of the CD150 family, which includes also e.g. CD48 and CD224. Receptors of this family regulate cytokine production and cytotoxicity of lymphocytes and NK cells. High levels of CD229 are found on T and B cells, where its expression increases during their maturation. It is absent on granulocytes, bone marrow-derived dendritic cells, platelets and erythrocytes. CD229 has been also reported on mouse monocytes and NK cells. CD229 interacts homophilically through its N-terminal domain and localizes to the contact site between T cells and antigen presenting B cells during antigen-dependent immune synapse formation.
- Pathways
- BCR Signaling