LY9 Antikörper
Kurzübersicht für LY9 Antikörper (ABIN487455)
Target
Alle LY9 (CD229) Antikörper anzeigenReaktivität
Wirt
Klonalität
Konjugat
Applikation
Klon
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Spezifität
- This antibody reacts with Human Ly-9 antigen.
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Kreuzreaktivität (Details)
- Species reactivity (tested):Human.
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Produktmerkmale
- Synonyms: Ly-9, T-lymphocyte surface antigen Ly-9, Lymphocyte antigen 9
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Immunogen
- Mouse pre-B-cell line 300. 19, stably transfected with the full length Human Ly-9 cDNA. Remarks: This antibody was concentrated from Hybridoma (clone HLy-9. 1. 25) supernatant. Hybridoma was established by fusion of mouse myeloma cell NS-1 with Balb/c mousesplenocyte.
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Isotyp
- IgG1
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Applikationshinweise
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Flow Cytometry: 10 μg/mLPositive Control: Peripheral blood T cells.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user. -
Protokoll
- Flow Cytometric Analysis for Floating CellsWe use Fisher tubes or equivalents as reaction tubes for all step described below. 1) Wash the cells 3 times with washing buffer [PBS containing 2% fetal calf serum (FCS).
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Beschränkungen
- Nur für Forschungszwecke einsetzbar
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Konzentration
- 1.0 mg/mL
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Buffer
- PBS, 7.2 containing 50 % Glycerol without preservatives.
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Konservierungsmittel
- Without preservative
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Lagerung
- 4 °C
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Informationen zur Lagerung
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Store the antibody undiluted at 2-8 °C.
Shelf life: one year from despatch. -
Haltbarkeit
- 12 months
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- LY9 (CD229) (Lymphocyte Antigen 9 (CD229))
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Andere Bezeichnung
- CD229 / LY9
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Hintergrund
- CD229 is a 100-120 kD cell surface glycoprotein that is the homologue of murine Ly-9. CD229 is a member of the immunoglobulin superfamily and may have a function in cell signalling. The antigen is expressed at low levels by mature T and B lymphocytes and at higher levels by single-positive thymocytes. Expression at variable levels is also seen in some cell lines, such as Daudi, U937 and HUT78.Synonyms: Ly-9, Lymphocyte antigen 9, T-lymphocyte surface antigen Ly-9
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Gen-ID
- 4063
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UniProt
- Q9HBG7
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Pathways
- BCR Signaling
Target
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