TNF alpha Antikörper (Biotin)

Produktnummer ABIN465814

Der Biotin-konjugierte Ziege Polyklonal anti-TNF alpha Antikörper (ABIN465814) detektiert spezifisch TNF alpha in WB und ELISA. Dieser Antikörper reagiert spezifisch mit Proben aus Human.

Kurzübersicht für TNF alpha Antikörper (Biotin) (ABIN465814)

Target: TNF alpha (Tumor Necrosis Factor alpha (TNF alpha))

Reaktivität: Human

Wirt: Ziege

Klonalität: Polyklonal

Konjugat: Dieser TNF alpha Antikörper ist konjugiert mit Biotin

Applikation: Western Blotting (WB), ELISA

Produktdetails anti-TNF alpha Antikörper (Biotin)

Spezifität: Human TNF-alpha

Aufreinigung: Immunoaffinity purified

Immunogen: E.coli derived recombinant Human TNF-alpha.

Type of Immunogen: Cell extract

Anwendungsinformationen

Applikationshinweise: Approved: ELISA (1 μg/mL), SwELISA, WB (0.1 - 0.2 μg/mL)

Usage: ELISA: To detect Human TNF-alpha by direct ELISA (using 100 μL/well antibody solution) a concentration of 0.25-1 μg/mL of this antibody is required. This biotinylated polyclonal antibody, in conjunction with compatible secondary reagents, allows the detection of at least 0.2-0.4 ng/well of recombinant Human TNF-alpha. To detect Human TNF-alpha by sandwich ELISA (using 100 μL/well antibody solution) a concentration of 0.25-1 μg/mL of this antibody is required. This biotinylated polyclonal antibody, in conjunction with Polyclonal Anti-Human TNF-alpha (LS-C104718) as a capture antibody, allows the detection of at least 0.2-0.4 ng/well of recombinant Human TNF-alpha. Western Blot: To detect Human TNF-alpha by Western Blot analysis this antibody can be used at a concentration of 0.1-0.2 μg/mL. When used in conjunction with compatible secondary reagents, the detection limit for recombinant Human TNF-alpha is 1.5-3 ng/lane, under either reducing or non-reducing conditions. The applications listed have been tested for the unconjugated form of this product. Other forms have not been tested.

Kommentare:

Target Species of Antibody: Human

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Lyophilized

Rekonstitution: Sterile PBS containing 0.1 % BSA

Konzentration: Lot specific

Buffer: Lyophilized from PBS,  pH 7.2

Handhabung: Avoid freeze/thaw cycles.

Lagerung: 4 °C,-20 °C

Informationen zur Lagerung: Store Lyophilized at room temperature up to 1 month
Reconstituted for up to 2 weeks at 2-8°C. Aliquot and freeze at -20°C for long term storage. Avoid freeze/thaw cycles.

Details zu TNF alpha

Target: TNF alpha (Tumor Necrosis Factor alpha (TNF alpha))

Andere Bezeichnung: TNF Alpha

Hintergrund: Name/Gene ID: TNF
Family: TNF

Synonyms: TNF, APC1 protein, Cachectin, DIF, TNF Alpha, Tumor necrosis factor-alpha, TNF-alpha, TNF-a, TNFA, Tumor necrosis factor, TNF, macrophage-derived, TNF, monocyte-derived, TNFSF2

Gen-ID: 7124

UniProt: P01375

Pathways: NF-kappaB Signalweg, Apoptose, Caspase Kaskade in der Apoptose, TLR Signalweg, Cellular Response to Molecule of Bacterial Origin, Regulation of Leukocyte Mediated Immunity, Positive Regulation of Immune Effector Process, Production of Molecular Mediator of Immune Response, Positive Regulation of Endopeptidase Activity, Hepatitis C, Protein targeting to Nucleus, Inflammasome