TNF alpha Antikörper

Produktnummer ABIN465030

Der Kaninchen Polyklonal anti-TNF alpha Antikörper (ABIN465030) detektiert spezifisch TNF alpha in WB, ELISA und Neut. Dieser Antikörper reagiert spezifisch mit Proben aus Human.

Kurzübersicht für TNF alpha Antikörper (ABIN465030)

Target: TNF alpha (Tumor Necrosis Factor alpha (TNF alpha))

Reaktivität: Human

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Dieser TNF alpha Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), ELISA, Neutralization (Neut)

Produktdetails anti-TNF alpha Antikörper

Spezifität: Human TNF-alpha

Aufreinigung: Immunoaffinity purified

Immunogen: E.coli derived recombinant Human TNF-alpha.

Type of Immunogen: Cell extract

Anwendungsinformationen

Applikationshinweise: Approved: ELISA (0.5 - 2 μg/mL), Neut (12 - 14 μg/mL), WB

Usage: Immunohistochemistry: This antibody stained formalin-fixed, paraffin-embedded sections of human breast invasive ductal carcinoma. The recommended concentrations are 0.5 μg/mL-1 μg/mL with an overnight incubation at 4 °C. An HRP-labeled polymer detection system was used with a DAB chromogen. Heat induced antigen retrieval with a  pH 6 Sodium Citrate buffer is recommended. Optimal concentrations and conditions may vary. *Additional Immunostaining data available. Please contact Tech Support for information. Neutralization: To yield one-half maximal inhibition [ND] of the biological activity of Human TNF-alpha (0.5 ng/mL), a concentration of 0.08-0.10 μg/mL of this antibody is required. ELISA: To detect Human TNF-alpha by sandwich ELISA (using 100 μL/well antibody solution) a concentration of 0.5-2 μg/mL of this antibody is required. This antigen affinity purified antibody, in conjunction with Biotinylated Anti-Human TNF-alpha (LS-C104719) as a detection antibody, allows the detection of at least 0.2-0.4 ng/well of recombinant Human TNF-alpha. Western Blot: To detect Human TNF-alpha by Western Blot analysis this antibody can be used at a concentration of 0.1-0.2 μg/mL. When used in conjunction with compatible secondary reagents, the detection limit for recombinant Human TNF-alpha is 1.5-3 ng/lane, under either reducing or non-reducing conditions.

Not recommended for: SwELISA

Kommentare:

Target Species of Antibody: Human

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Lyophilized

Rekonstitution: Sterile water

Konzentration: Lot specific

Buffer: Lyophilized from PBS,  pH 7.2

Handhabung: Avoid freeze/thaw cycles.

Lagerung: 4 °C,-20 °C

Informationen zur Lagerung: Store Lyophilized at room temperature up to 1 month
Reconstituted for up to 2 weeks at 2-8°C. Aliquot and freeze at -20°C for long term storage. Avoid freeze/thaw cycles.

Details zu TNF alpha

Target: TNF alpha (Tumor Necrosis Factor alpha (TNF alpha))

Andere Bezeichnung: TNF Alpha

Hintergrund: Name/Gene ID: TNF
Family: TNF

Synonyms: TNF, APC1 protein, Cachectin, DIF, TNF Alpha, Tumor necrosis factor-alpha, TNF-alpha, TNF-a, TNFA, Tumor necrosis factor, TNF, macrophage-derived, TNF, monocyte-derived, TNFSF2

Gen-ID: 7124

UniProt: P01375

Pathways: NF-kappaB Signalweg, Apoptose, Caspase Kaskade in der Apoptose, TLR Signalweg, Cellular Response to Molecule of Bacterial Origin, Regulation of Leukocyte Mediated Immunity, Positive Regulation of Immune Effector Process, Production of Molecular Mediator of Immune Response, Positive Regulation of Endopeptidase Activity, Hepatitis C, Protein targeting to Nucleus, Inflammasome