SPHK2 Antikörper (N-Term)

Produktnummer ABIN391348

Dieses Kaninchen Polyklonal-Antikörper erkennt spezifisch SPHK2 in WB und IHC (p). Er zeigt eine Reaktivität gegenüber Human und wurde in 8+ Publikationen erwähnt.

Kurzübersicht für SPHK2 Antikörper (N-Term) (ABIN391348)

Target: SPHK2 (Sphingosine Kinase 2 (SPHK2))

Reaktivität: Human

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Dieser SPHK2 Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Klon: RB03623

Produktdetails anti-SPHK2 Antikörper

Bindungsspezifität: AA 1-30, N-Term

Aufreinigung: This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS.

Immunogen: This SPHK2 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 1-30 amino acids from the N-terminal region of human SPHK2.

Isotyp: Ig Fraction

Anwendungsinformationen

Applikationshinweise: WB: 1:1000. IHC-P: 1:50~100

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Buffer: Purified polyclonal antibody supplied in PBS with 0.09 % (W/V) sodium azide.

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Lagerung: 4 °C,-20 °C

Informationen zur Lagerung: Maintain refrigerated at 2-8 °C for up to 6 months. For long term storage store at -20 °C.

Haltbarkeit: 6 months

Referenzen für anti-SPHK2 Antikörper (ABIN391348)

Song, Zhang, Chen, Xia, Qin, Waeber, Sheng: "Sphingosine kinase 2 activates autophagy and protects neurons against ischemic injury through interaction with Bcl-2 via its putative BH3 domain." in: Cell death & disease, Vol. 8, Issue 7, pp. e2912, (2018) (PubMed).

Gairhe, Joshi, Bastola, McLendon, Oka, Fagan, McMurtry: "Sphingosine-1-phosphate is involved in the occlusive arteriopathy of pulmonary arterial hypertension." in: Pulmonary circulation, Vol. 6, Issue 3, pp. 369-80, (2016) (PubMed).

Sun, Zhang, Wang, Wang, Ma, Lei, Zhou, Sun, Lu, Liu, Han: "Down-regulation of Sphk2 suppresses bladder cancer progression." in: Tumour biology, (2015) (PubMed).

Sheng, Zhang, Felice, Qin, Qin, Smith, Sapp, Difiglia, Waeber: "Preconditioning stimuli induce autophagy via sphingosine kinase 2 in mouse cortical neurons." in: The Journal of biological chemistry, Vol. 289, Issue 30, pp. 20845-57, (2014) (PubMed).

Candela, Geraci, Turturici, Taverna, Albanese, Sconzo: "Membrane vesicles containing matrix metalloproteinase-9 and fibroblast growth factor-2 are released into the extracellular space from mouse mesoangioblast stem cells." in: Journal of cellular physiology, Vol. 224, Issue 1, pp. 144-51, (2010) (PubMed).

Weigert, Cremer, Schmidt, von Knethen, Angioni, Geisslinger, Brüne: "Cleavage of sphingosine kinase 2 by caspase-1 provokes its release from apoptotic cells." in: Blood, Vol. 115, Issue 17, pp. 3531-40, (2010) (PubMed).

Wadgaonkar, Patel, Grinkina, Romano, Liu, Zhao, Sammani, Garcia, Natarajan: "Differential regulation of sphingosine kinases 1 and 2 in lung injury." in: American journal of physiology. Lung cellular and molecular physiology, Vol. 296, Issue 4, pp. L603-13, (2009) (PubMed).

Coste, Brenneis, Linke, Pierre, Maeurer, Becker, Schmidt, Gao, Geisslinger, Scholich: "Sphingosine 1-phosphate modulates spinal nociceptive processing." in: The Journal of biological chemistry, Vol. 283, Issue 47, pp. 32442-51, (2008) (PubMed).

Details zu SPHK2

Target: SPHK2 (Sphingosine Kinase 2 (SPHK2))

Andere Bezeichnung: SPHK2

Hintergrund: Sphingosine Kinase (SphK) catalyzes the phosphorylation of the lipid sphingosine, creating the bioactive lipid sphingosine-1-phosphate (S1P). S1P subsequently signals through cell surface G protein-coupled receptors, as well as intracellularly, to modulate cell proliferation, survival, motility and differentiation. SphK is an important signaling enzyme which is activated by diverse agents, including growth factors that signal through receptor tyrosine kinases, agents activating G protein-coupled receptors, and immunoglobulin receptors. Two SphK isotypes, SphK-1 and SphK-2, have been cloned, and both isotypes are ubiquitously expressed. SphK-1 has been shown to mediate cell growth, prevention of apoptosis, and cellular transformation, and is upregulated in a variety of human tumors. In contrast, SphK-2 increases apoptosis, and may be responsible for phosphorylating and activating the immunosuppressive drug FTY720.

Molekulargewicht: 69217

NCBI Accession: NP_001191087, NP_001191088, NP_001191089, NP_064511

UniProt: Q9NRA0

Pathways: VEGF Signaling