SPHK2 Antikörper (C-Term)

Produktnummer ABIN391349

Es sind 2+ Publikationen für dieses Produkt verfügbar. Der Kaninchen Polyklonal anti-SPHK2 Antikörper wird verwendet zum Nachweis von SPHK2 in Proben von Human. Er wurde validiert für WB und IHC (p).

Kurzübersicht für SPHK2 Antikörper (C-Term) (ABIN391349)

Target: SPHK2 (Sphingosine Kinase 2 (SPHK2))

Reaktivität: Human

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Dieser SPHK2 Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Klon: RB03625

Produktdetails anti-SPHK2 Antikörper

Bindungsspezifität: AA 590-620, C-Term

Aufreinigung: This antibody is purified through a protein A column, followed by peptide affinity purification.

Immunogen: This SPHK2 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 590-620 amino acids from the C-terminal region of human SPHK2.

Isotyp: IgG

Anwendungsinformationen

Applikationshinweise: WB: 1:1000. WB: 1:1000. IHC-P-Leica: 1:250. IHC-P-Leica: 1:250

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Buffer: Purified polyclonal antibody supplied in PBS with 0.09 % (W/V) sodium azide.

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handhabung: Avoid freeze-thaw cycles.

Lagerung: 4 °C,-20 °C

Informationen zur Lagerung: Maintain refrigerated at 2-8 °C for up to 6 months. For long term storage store at -20 °C in small aliquots.

Haltbarkeit: 6 months

Referenzen für anti-SPHK2 Antikörper (ABIN391349)

Sun, Zhang, Wang, Wang, Ma, Lei, Zhou, Sun, Lu, Liu, Han: "Down-regulation of Sphk2 suppresses bladder cancer progression." in: Tumour biology, (2015) (PubMed).

Coste, Brenneis, Linke, Pierre, Maeurer, Becker, Schmidt, Gao, Geisslinger, Scholich: "Sphingosine 1-phosphate modulates spinal nociceptive processing." in: The Journal of biological chemistry, Vol. 283, Issue 47, pp. 32442-51, (2008) (PubMed).

Details zu SPHK2

Target: SPHK2 (Sphingosine Kinase 2 (SPHK2))

Andere Bezeichnung: SPHK2

Hintergrund: Sphingosine Kinase (SphK) catalyzes the phosphorylation of the lipid sphingosine, creating the bioactive lipid sphingosine-1-phosphate (S1P). S1P subsequently signals through cell surface G protein-coupled receptors, as well as intracellularly, to modulate cell proliferation, survival, motility and differentiation. SphK is an important signaling enzyme which is activated by diverse agents, including growth factors that signal through receptor tyrosine kinases, agents activating G protein-coupled receptors, and immunoglobulin receptors. Two SphK isotypes, SphK-1 and SphK-2, have been cloned, and both isotypes are ubiquitously expressed. SphK-1 has been shown to mediate cell growth, prevention of apoptosis, and cellular transformation, and is upregulated in a variety of human tumors. In contrast, SphK-2 increases apoptosis, and may be responsible for phosphorylating and activating the immunosuppressive drug FTY720.

Molekulargewicht: 69217

NCBI Accession: NP_001191087, NP_001191088, NP_001191089, NP_064511

UniProt: Q9NRA0

Pathways: VEGF Signaling