c-MET Antikörper (C-Term)

Produktnummer ABIN375293

Der Kaninchen Polyklonal Anti-c-MET-Antikörper wurde für WB, ELISA und IHC validiert. Er ist geeignet, c-MET in Proben von Human zu detektieren.

Kurzübersicht für c-MET Antikörper (C-Term) (ABIN375293)

Target: c-MET (MET) (Met Proto-Oncogene (MET))

Reaktivität: Human

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Dieser c-MET Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), ELISA, Immunohistochemistry (IHC)

Produktdetails anti-c-MET Antikörper

Bindungsspezifität: C-Term

Spezifität: Reacts with human 155 kDa HGF receptor and cleaved c-Met

Kreuzreaktivität (Details): Cross reacts with HGF receptor from many species due to sequence homology.

Aufreinigung: Antiserum

Immunogen: Synthetic peptide derived from Cter domain of human HGF receptor

Isotyp: IgG

Anwendungsinformationen

Applikationshinweise:

Working dilution: Optimal dilution should be determined by the end user.
The following are guidelines only :
- IHC : 1/100 to 1/1 000 - WB : 1/1 000 to 1/5 000

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Lyophilized

Rekonstitution: Must be reconstituted in distilled water.

Lagerung: 4 °C/-20 °C

Informationen zur Lagerung: Lyophilized powder stable for a minimum of 2 years at -20°C. Store reconstituted antibodies at +4°C. For extended periods store in aliquots at -20°C. Antibodies are guaranteed for 6 month from date of receipt.

Haltbarkeit: 24 months

Details zu c-MET

Target: c-MET (MET) (Met Proto-Oncogene (MET))

Andere Bezeichnung: Hepatocyte Growth Factor Receptor (HGF Receptor)

Hintergrund: Receptor tyrosine kinase that transduces signals from the extracellular matrix into the cytoplasm by binding to hepatocyte growth factor/HGF ligand. Regulates many physiological processes including proliferation, scattering, morphogenesis and survival. Ligand binding at the cell surface induces autophosphorylation of MET on its intracellular domain that provides docking sites for downstream signaling molecules. Following activation by ligand, interacts with the PI3-kinase subunit PIK3R1, PLCG1, SRC, GRB2, STAT3 or the adapter GAB1. Recruitment of these downstream effectors by MET leads to the activation of several signaling cascades including the RAS-ERK, PI3 kinase-AKT, or PLCgamma-PKC. The RAS-ERK activation is associated with the morphogenetic effects while PI3K/AKT coordinates prosurvival effects. During embryonic development, MET signaling plays a role in gastrulation, development and migration of muscles and neuronal precursors, angiogenesis and kidney formation. In adults, participates in wound healing as well as organ regeneration and tissue remodeling. Promotes also differentiation and proliferation of hematopoietic cells.

Pathways: RTK Signalweg, Carbohydrate Homeostasis, Synaptic Membrane, Signaling of Hepatocyte Growth Factor Receptor