CD14 Antikörper (Internal Region)

Produktnummer ABIN374645

Der Ziege Polyklonal anti-CD14 Antikörper wird verwendet zum Nachweis von CD14 in Proben von Human. Er wurde validiert für WB, IHC (p) und EIA.

Kurzübersicht für CD14 Antikörper (Internal Region) (ABIN374645)

Target: CD14

Reaktivität: Human

Wirt: Ziege

Klonalität: Polyklonal

Konjugat: Dieser CD14 Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Enzyme Immunoassay (EIA)

Produktdetails anti-CD14 Antikörper

Bindungsspezifität: Internal Region

Sequenz: C-KRVDADADPR QYAD

Spezifität: This antibody recognizes CD14.

Kreuzreaktivität (Details): Species reactivity (tested):Human.

Aufreinigung: Ammonium Sulphate Precipitation followed by Antigen Affinity Chromatography using the immunizing peptide.

Immunogen: Peptide with sequence from the internal region of the protein sequence according to NP_000582.1. Genename: CD14

Anwendungsinformationen

Applikationshinweise: Peptide ELISA: 1/128000. Western Blot: 1 - 3 μg/mL. Immunohistochemistry on paraffin sections: 4 - 6 μg/mL.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Konzentration: 0.5 mg/mL

Buffer: Tris saline, pH ~7.3 containing 0.02 % Sodium Azide as preservative and 0.5 % BSA as stabilizer.

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handhabung: Avoid repeated freezing and thawing.

Lagerung: 4 °C/-20 °C

Informationen zur Lagerung: Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer.

Details zu CD14

Target: CD14

Andere Bezeichnung: CD14

Hintergrund: CD14 is a single copy gene encoding 2 protein forms: a 50 to 55 kDa glycosylphosphatidylinositol anchored membrane protein (mCD14) and a monocyte or liver derived soluble serum protein (sCD14) that lacks the anchor. Both molecules are critical for lipopolysaccharide (LPS) dependent signal transduction, and sCD14 confers LPS sensitivity to cells lacking mCD14. Increased sCD14 levels are associated with inflammatory infectious diseases and high mortality in gram negative shock. CD14 also appears to be involved in clearance of gram-negative bacteria via its high affinity binding to LPS-LPB complexes.Synonyms: Monocyte differentiation antigen CD14, Myeloid cell-specific leucine-rich glycoprotein

Gen-ID: 929, 9606

UniProt: P08571

Pathways: TLR Signalweg, Activation of Innate immune Response, Cellular Response to Molecule of Bacterial Origin, Toll-Like Receptors Cascades