NPM1 Antikörper (C-Term)

Produktnummer ABIN374536

Dieser Ziege Polyklonal Antikörper detektiert spezifisch NPM1 in WB und EIA. Es zeigt Reaktivität gegenüber Proben von Human, Maus, Ratte, Hund und Schimpanse.

Kurzübersicht für NPM1 Antikörper (C-Term) (ABIN374536)

Target: NPM1 (Nucleophosmin (Nucleolar phosphoprotein B23, Numatrin) (NPM1))

Reaktivität: Human, Maus, Ratte, Hund, Schimpanse

Wirt: Ziege

Klonalität: Polyklonal

Konjugat: Dieser NPM1 Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), Enzyme Immunoassay (EIA)

Produktdetails anti-NPM1 Antikörper

Bindungsspezifität: C-Term

Sequenz: C-QEAIQDLWQW RKSL

Spezifität: This antibody recognizes Nucleophosmin.

Kreuzreaktivität (Details): Species reactivity (expected):Canine, Bovine.
Species reactivity (tested):Human, Mouse, Rat.

Aufreinigung: Ammonium Sulphate Precipitation followed by antigen Affinity Chromatography using the immunizing peptide

Immunogen: Peptide with sequence from the C Terminus of the protein sequence according to NP_002511.1, NP_954654.1.

Anwendungsinformationen

Applikationshinweise: Peptide ELISA: 1/2000. Western Blot: 0.1 - 0.3 μg/mL.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Konzentration: 0.5 mg/mL

Buffer: Tris saline, pH ~7.3 containing 0.02 % Sodium Azide and 0.5 % BSA

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handhabung: Avoid repeated freezing and thawing.

Lagerung: 4 °C/-20 °C

Informationen zur Lagerung: Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer.

Details zu NPM1

Target: NPM1 (Nucleophosmin (Nucleolar phosphoprotein B23, Numatrin) (NPM1))

Andere Bezeichnung: Nucleophosmin

Hintergrund: Nucleophosmin (NPM) also called B23, nutramin and NO38 is a ubiquitously expressed phosphoprotein involved in ribosome assembly/transport, cytoplasmic/nuclear trafficking, regulation of DNA polymerase alpha activity, centrosome duplication, and regulation of p53. It is involved in the acute response of mammalian cells to environmental stress, such as UV rays. NPM continuously shuttles between the nucleus and cytoplasm. It has been shown to bind nucleic acid, prevent protein aggregation via its chaperone activities, protect enzymes during thermal denaturation, and facilitate renaturation of chemically denatured proteins. In its cellular structure role, there is evidence suggesting NPM is associated with the centrosome. It is the substrate of CDK2/cyclin E during duplication of centrosomes (cellular division). Because of NPM gene interaction with several tumor-associated chromosome translocations, NPM is thought to be a portion of several fusion proteins: NPM-ALK, NPM-RAR, and NPM-MLF1. While it is not thought to be part of the transforming potential of these fusion proteins, it is believed to act as the interface for oligomerization and oncogenic conversion of these tumor promoting fusion proteins.Synonyms: NPM, NPM1, Nucleolar Phosphoprotein B23, Nucleolar Protein NO38, Numatrin

Gen-ID: 4869

NCBI Accession: NP_002511

UniProt: P06748

Pathways: Ribonucleoprotein Complex Subunit Organization, Ribosome Assembly