Rekombinanter NPM1 Antikörper (AA 185-287)

Produktnummer ABIN6940222

Dieser Maus Monoklonal Antikörper detektiert spezifisch NPM1 in WB, IHC und FACS. Es zeigt Reaktivität gegenüber Proben von Human.

Kurzübersicht für Rekombinanter NPM1 Antikörper (AA 185-287) (ABIN6940222)

Target: NPM1 (Nucleophosmin (Nucleolar phosphoprotein B23, Numatrin) (NPM1))

Antikörpertyp: Recombinant Antibody

Reaktivität: Human

Wirt: Maus

Klonalität: Monoklonal

Konjugat: Dieser NPM1 Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), Immunohistochemistry (IHC), Flow Cytometry (FACS)

Klon: RNPM1-1901

Produktdetails anti-NPM1 Antikörper

Bindungsspezifität: AA 185-287

Verwendungszweck: Recombinant Nucleophosmin (Acute Myeloid Leukemia Marker) Antibody

Spezifität: Recognizes a 33 kDa glycoprotein, identified as Nucleophosmin (NPM). It is predominantly localized in the nucleus of cells in most tissues. NPM is involved in ribosomal assembly and rRNA transport. It is an abundant protein that is highly phosphorylated by Cdc2 kinase during mitosis. This phosphoprotein moves between the nucleus and the cytoplasm. It is thought to be involved in several processes including regulation of the ARF/p53 pathway. A number of genes are fusion partners, in particular the anaplastic lymphoma kinase gene on chromosome 2. Mutations in exon 12 affecting the C-terminus of the protein are associated with an aberrant cytoplasmic location. Mutations in this gene are associated with acute myeloid leukemia.The antibody may be a useful aid for classification of acute myeloid leukemia.

Aufreinigung: 200ug/ml of Ab Purified from Bioreactor Concentrate by Protein A/G.

Immunogen: Recombinant human NPM1 protein fragment (aa185-287) (exact sequence is proprietary)

Isotyp: IgG1, kappa

Anwendungsinformationen

Applikationshinweise:

Positive Control: MCF-7, HeLa or A431 cell lysate (WB). Human skin or colon tissue (IHC).

Known Application: Immunohistochemistry (Formalin-fixed. Not suitable for frozen tissues.) (0.5-1 μg/mL for 30 minutes at RT)(Staining of formalin-fixed tissues requires boiling tissue sections in 10 mM citrate buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 minutes)Optimal dilution for a specific application should be determined.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Konzentration: 200 μg/mL

Buffer: Prepared in 10 mM PBS with 0.05 % BSA & 0.05 % azide.

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Lagerung: 4 °C

Informationen zur Lagerung: Antibody with azide - store at 2 to 8°C. Antibody without azide - store at -20 to -80°C. Antibody is stable for 24 months. Non-hazardous. No MSDS required.

Haltbarkeit: 24 months

Details zu NPM1

Target: NPM1 (Nucleophosmin (Nucleolar phosphoprotein B23, Numatrin) (NPM1))

Andere Bezeichnung: NPM1

Hintergrund:

NO38, NPM, NPM1, Nucleolar phosphoprotein B23, Nucleolar protein NO38, Nucleophosmin (nucleolar phosphoprotein B23 numatrin), Nucleophosmin, Nucleophosmin/nucleoplasmin family member 1, Numatrin

HeLa or A431 cells (WB/FC). Human skin or colon tissue (IHC)., MCF-7, Pancreas.

Molekulargewicht: 33kDa

Gen-ID: 4869, 557550

UniProt: P06748

Pathways: Ribonucleoprotein Complex Subunit Organization, Ribosome Assembly