NPM1 Antikörper (C-Term)

Produktnummer ABIN185372

Der Ziege Polyklonal anti-NPM1 Antikörper wird verwendet zum Nachweis von NPM1 in Proben von Human und Maus. Er wurde validiert für WB, IHC, IF und ELISA.

Kurzübersicht für NPM1 Antikörper (C-Term) (ABIN185372)

Target: NPM1 (Nucleophosmin (Nucleolar phosphoprotein B23, Numatrin) (NPM1))

Reaktivität: Human, Maus

Wirt: Ziege

Klonalität: Polyklonal

Konjugat: Dieser NPM1 Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), Immunohistochemistry (IHC), Immunofluorescence (IF), ELISA

Güteklasse: Verified

Produktdetails anti-NPM1 Antikörper

Bindungsspezifität: C-Term

Verwendungszweck: Nucleophosmin / NPM1

Sequenz: QEAIQDLWQW RKSL

Kreuzreaktivität: Rind (Kuh), Hund, Human, Maus, Ratte

Aufreinigung: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Immunogen: Peptide with sequence C-QEAIQDLWQWRKSL, from the C Terminus of the protein sequence according to NP_002511.1, NP_954654.1.

Isotyp: IgG

Anwendungsinformationen

Applikationshinweise:

Immunohistochemistry: Paraffin embedded Human Testis. Recommended concentration: 5 μg/mL.

Western Blot: Approx 37 kDa band observed in lysates of cell lines Daudi, Jurkat, K562 and NIH3T3 and in Mouse Spleen lysates (calculated MW of 32.6 kDa according to Human NP_001341935.1 and Mouse NP_032748.1). This molecular weight is routinely observed b

Peptide ELISA: antibody detection limit dilution 1:8000.

Kommentare:

Immunofluorescence: Strong expression of the protein seen in the nucleoli of HeLa cells. Recommended concentration: 10μg/ml.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Konzentration: 0.5 mg/mL

Buffer: Supplied at 0.5 mg/mL in Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin.

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handhabung: Minimize freezing and thawing.

Lagerung: -20 °C

Informationen zur Lagerung: Aliquot and store at -20°C, with minimal freeze/thawing. A working aliquot may be refrigerated at 4°C for a few weeks and still remain viable.

Details zu NPM1

Target: NPM1 (Nucleophosmin (Nucleolar phosphoprotein B23, Numatrin) (NPM1))

Andere Bezeichnung: NPM1

Hintergrund: NPM1, NPM, B23, nucleophosmin (nucleolar phosphoprotein B23, numatrin), numatrin, nucleolar phosphoprotein B23, nucleophosmin/nucleoplasmin family, member 1, MGC104254, nucleophosmin 1

Gen-ID: 4869

NCBI Accession: NP_001341935, NP_954654, NP_001341936, NP_001341939

Pathways: Ribonucleoprotein Complex Subunit Organization, Ribosome Assembly