NPM1 Antikörper (pThr199)

Produktnummer ABIN6256499

Produktdetails anti-NPM1 Antikörper

Target: NPM1 (Nucleophosmin (Nucleolar phosphoprotein B23, Numatrin) (NPM1))

Bindungsspezifität: pThr199

Reaktivität: Human, Maus, Ratte

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Dieser NPM1 Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), Immunohistochemistry (IHC), ELISA, Immunofluorescence (IF), Immunocytochemistry (ICC)

Spezifität: Phospho-Nucleophosmin (Thr199) Antibody detects endogenous levels of Nucleophosmin only when phosphorylated at Threonine 199.

Homologie: Pig,Bovine,Horse,Sheep,Rabbit,Dog

Aufreinigung: The antibody is from purified rabbit serum by affinity purification via sequential chromatography on phospho- and non-phospho-peptide affinity columns.

Immunogen: A synthesized peptide derived from human Nucleophosmin around the phosphorylation site of Thr199.

Isotyp: IgG

Anwendungsinformationen

Applikationshinweise: WB 1:500-1:2000, IHC 1:50-1:200, IF/ICC 1:100-1:500, ELISA(peptide) 1:20000-1:40000

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Konzentration: 1 mg/mL

Buffer: Rabbit IgG in phosphate buffered saline , pH 7.4, 150 mM NaCl, 0.02 % sodium azide and 50 % glycerol.

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Lagerung: -20 °C

Informationen zur Lagerung: Store at -20 °C. Stable for 12 months from date of receipt.

Haltbarkeit: 12 months

Details zu NPM1

Target: NPM1 (Nucleophosmin (Nucleolar phosphoprotein B23, Numatrin) (NPM1))

Andere Bezeichnung: NPM1 (NPM1 Produkte)

Synonyme: B23 antikoerper, NPM antikoerper, NO38 antikoerper, Npm antikoerper, B23NP antikoerper, npm4 antikoerper, npm antikoerper, CG7917 antikoerper, CRP1 antikoerper, Crp1 antikoerper, Dmel\\CG7917 antikoerper, dNLP antikoerper, dNLP-S antikoerper, p22 antikoerper, npm1 antikoerper, sb:cb487 antikoerper, nucleophosmin 1 antikoerper, nucleophosmin (nucleolar phosphoprotein B23, numatrin) L homeolog antikoerper, nucleophosmin 1b antikoerper, Nucleophosmin antikoerper, nucleophosmin antikoerper, nucleophosmin (nucleolar phosphoprotein B23, numatrin) antikoerper, Nucleoplasmin antikoerper, nucleophosmin 1a antikoerper, NPM1 antikoerper, Npm1 antikoerper, npm1.L antikoerper, npm1b antikoerper, npm antikoerper, Nlp antikoerper, npm1a antikoerper

Hintergrund:

Description: Involved in diverse cellular processes such as ribosome biogenesis, centrosome duplication, protein chaperoning, histone assembly, cell proliferation, and regulation of tumor suppressors p53/TP53 and ARF. Binds ribosome presumably to drive ribosome nuclear export. Associated with nucleolar ribonucleoprotein structures and bind single-stranded nucleic acids. Acts as a chaperonin for the core histones H3, H2B and H4. Stimulates APEX1 endonuclease activity on apurinic/apyrimidinic (AP) double-stranded DNA but inhibits APEX1 endonuclease activity on AP single-stranded RNA. May exert a control of APEX1 endonuclease activity within nucleoli devoted to repair AP on rDNA and the removal of oxidized rRNA molecules. In concert with BRCA2, regulates centrosome duplication. Regulates centriole duplication: phosphorylation by PLK2 is able to trigger centriole replication. Negatively regulates the activation of EIF2AK2/PKR and suppresses apoptosis through inhibition of EIF2AK2/PKR autophosphorylation. Antagonizes the inhibitory effect of ATF5 on cell proliferation and relieves ATF5-induced G2/M blockade (PubMed:22528486). In complex with MYC enhances the transcription of MYC target genes (PubMed:25956029).

Gene: NPM1

Molekulargewicht: 32/38kDa

Gen-ID: 4869

UniProt: P06748

Pathways: Ribonucleoprotein Complex Subunit Organization, Ribosome Assembly