Telefon:
+49 (0)241 95 163 153
Fax:
+49 (0)241 95 163 155
E-Mail:
orders@antikoerper-online.de

PYCARD Antikörper (C-Term)

PYCARD Reaktivität: Human WB, IHC (p), EIA Wirt: Ziege Polyclonal unconjugated
Produktnummer ABIN374154
  • Target Alle PYCARD Antikörper anzeigen
    PYCARD (PYD and CARD Domain Containing (PYCARD))
    Bindungsspezifität
    • 10
    • 6
    • 6
    • 5
    • 2
    • 2
    • 2
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    C-Term
    Reaktivität
    • 58
    • 10
    • 9
    • 1
    • 1
    • 1
    Human
    Wirt
    • 49
    • 7
    • 3
    Ziege
    Klonalität
    • 47
    • 12
    Polyklonal
    Konjugat
    • 34
    • 4
    • 3
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    Dieser PYCARD Antikörper ist unkonjugiert
    Applikation
    • 41
    • 23
    • 15
    • 14
    • 5
    • 5
    • 4
    • 4
    • 2
    • 1
    • 1
    Western Blotting (WB), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Enzyme Immunoassay (EIA)
    Sequenz
    C-RESQSYLVED LERS
    Spezifität
    This antibody is expected to recognize all reported Human isoforms of PYCARD according to NP_037390.2 and NP_660183.1.
    Kreuzreaktivität (Details)
    Species reactivity (tested):Human.
    Aufreinigung
    Ammonium Sulphate Precipitation followed by Antigen Affinity Chromatography using the immunizing peptide
    Immunogen
    Peptide from C Terminus of the protein sequence according to NP_037390.2, NP_660183.1 Genename: PYCARD
  • Applikationshinweise
    Peptide ELISA: Limit dilution 1: 32000. Western blot: 1-3 μg/mL. Approx.22 kDa band observed in Hela and Human Kidney lysates(predicted MW of 22 kDa according to NP_037390.2).
    Other applications not tested.
    Optimal dilutions are dependent on conditions and should be determined by the user.
    Beschränkungen
    Nur für Forschungszwecke einsetzbar
  • Konzentration
    0.5 mg/mL
    Buffer
    Tris saline, pH ~7.3 containing 0.02 % Sodium Azide as preservative and 0.5 % BSA as stabilizer
    Konservierungsmittel
    Sodium azide
    Vorsichtsmaßnahmen
    This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
    Handhabung
    Avoid repeated freezing and thawing.
    Lagerung
    4 °C/-20 °C
    Informationen zur Lagerung
    Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer.
  • Target
    PYCARD (PYD and CARD Domain Containing (PYCARD))
    Andere Bezeichnung
    CARD5 / PYCARD / ASC (PYCARD Produkte)
    Synonyme
    ASC antikoerper, CARD5 antikoerper, TMS antikoerper, TMS-1 antikoerper, TMS1 antikoerper, 9130417A21Rik antikoerper, Asc antikoerper, TNS1 antikoerper, masc antikoerper, asc1 antikoerper, zAsc antikoerper, zgc:109874 antikoerper, PYD and CARD domain containing antikoerper, PYCARD antikoerper, Pycard antikoerper, pycard antikoerper
    Hintergrund
    TMS1 (target of methylation-induced silencing) or ASC (apoptosis-associated speck-like protein containing a CARD) promotes caspase-mediated apoptosis. TMS1 is aberrantly methylated and silenced in human breast cancer cells, the methylation-mediated silencing of TMS1 may confer a survival advantage by allowing cells to escape from apoptosis, supporting a new role for aberrant methylation in breast tumorigenesis. TMS1 encodes a 22- kDa predicted protein containing a COOH-terminal caspase recruitment domain (CARD). However, it is structurally unrelated to other known CARD adaptor and regulatory proteins. Ectopic expression of TMS1 alone can trigger apoptosis in 293 cells, and cell death correlated with relocalization of ASC from the cytoplasm to perinuclear, ball-like structures.Synonyms: Caspase recruitment domain-containing protein 5, PYD and CARD domain-containing protein, TMS1, Target of methylation-induced silencing 1, poptosis-associated speck-like protein containing a CARD
    Gen-ID
    29108
    NCBI Accession
    NP_037390
    UniProt
    Q9ULZ3
    Pathways
    Activation of Innate immune Response, Cellular Response to Molecule of Bacterial Origin, Regulation of Actin Filament Polymerization, Positive Regulation of Endopeptidase Activity, Activated T Cell Proliferation, Inflammasome
Sie sind hier:
Kundenservice