SMAD4 Antikörper (C-Term)

Produktnummer ABIN374104

Dieser Ziege Polyklonal Antikörper detektiert spezifisch SMAD4 in WB und EIA. Es zeigt Reaktivität gegenüber Proben von Human, Maus, Ratte, Rind (Kuh), Hund und Schwein.

Kurzübersicht für SMAD4 Antikörper (C-Term) (ABIN374104)

Target: SMAD4 (SMAD Family Member 4 (SMAD4))

Reaktivität: Human, Maus, Ratte, Rind (Kuh), Hund, Schwein

Wirt: Ziege

Klonalität: Polyklonal

Konjugat: Dieser SMAD4 Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), Enzyme Immunoassay (EIA)

Produktdetails anti-SMAD4 Antikörper

Bindungsspezifität: C-Term

Sequenz: C-HTMPIADPQP LD

Spezifität: This antibody detects SMAD4 at C-term.

Kreuzreaktivität (Details): Species reactivity (expected):Human, Rat, Canine (Dog), Pig, Cow.
Species reactivity (tested):Mouse.

Aufreinigung: Ammonium Sulphate Precipitation followed by Antigen Affinity Chromatography using the immunizing peptide.

Immunogen: Peptide from C-Terminus of the protein sequence according to NP_005350.1 Genename: SMAD4

Anwendungsinformationen

Applikationshinweise: Peptide ELISA: Limit dilution 1: 32000. Western blot: 0.5-2 μg/mL. Approx 60-65 kDa band seen in A431 cell lysate.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Konzentration: 0.5 mg/mL

Buffer: Tris saline, pH 7.3 containing 0.02 % Sodium Azide as preservative and 0.5 % BSA as stabilizer.

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handhabung: Avoid repeated freezing and thawing.

Lagerung: 4 °C/-20 °C

Informationen zur Lagerung: Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer.

Details zu SMAD4

Target: SMAD4 (SMAD Family Member 4 (SMAD4))

Andere Bezeichnung: SMAD4

Hintergrund: SMAD4 (also known as Mothers against decapentaplegic homolog 4, Mothers against DPP homolog 4, deletion target in pancreatic carcinoma 4 and hSMAD4) is a common mediator of signal transduction by TGF-beta (transforming growth factor), but is also involved in cancer development and metastases as a tumor suppressor. SMAD4 promotes binding of the SMAD2/SMAD4/FAST-1 complex to DNA and provides an activation function required for SMAD1 or SMAD2 to stimulate transcription. SMAD4 may form trimers with receptor-regulated SMAD (R-SMAD) and interacts with ATF2, COPS5, DACH1, MSG1, SKI and TRIM33. In the absence of ligand SMAD4 is found in the cytoplasm, but when complexed with R-SMAD, translocates to the nucleus. Defects in SMAD4 are a cause of pancreatic carcinoma and juvenile polyposis syndrome (JPS), a syndrome in which patients are at risk for developing gastrointestinal cancers.Synonyms: DPC4, Deletion target in pancreatic carcinoma 4, MAD homolog 4, MADH4, Mothers against DPP homolog 4, Mothers against decapentaplegic homolog 4, SMAD 4, SMAD family member 4, SMAD-4

Gen-ID: 4089

NCBI Accession: NP_005350

UniProt: Q13485

Pathways: Zellzyklus, Chromatin Binding, Autophagie