MAPK14 Antikörper (pThr180, pTyr182)

Produktnummer ABIN372694

Dieses Kaninchen Polyklonal-Antikörper erkennt spezifisch MAPK14 in WB und IHC (fro). Er zeigt eine Reaktivität gegenüber Human, Maus, Ratte, Hund, Huhn, Rind (Kuh), Zebrafisch (Danio rerio) und Non-Human Primate und wurde in 1 Publikation erwähnt.

Kurzübersicht für MAPK14 Antikörper (pThr180, pTyr182) (ABIN372694)

Target: MAPK14 (Mitogen-Activated Protein Kinase 14 (MAPK14))

Reaktivität: Human, Maus, Ratte, Hund, Huhn, Rind (Kuh), Zebrafisch (Danio rerio), Non-Human Primate

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Dieser MAPK14 Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), Immunohistochemistry (Frozen Sections) (IHC (fro))

Produktdetails anti-MAPK14 Antikörper

Bindungsspezifität: pThr180, pTyr182

Spezifität: This antibody recognizes the ~39k p38 MAPK protein phosphorylated at Thr180/Tyr182. Immunolabeling is blocked by preadsorption of the antibody with the phosphopeptide used as antigen but not by the corresponding dephosphopeptide.

Kreuzreaktivität (Details): Species reactivity (expected):Bovine, Canine, Chicken, Mouse, Zebrafish and non-Human Primates.
Species reactivity (tested):Human.

Aufreinigung: Sequential Chromatography on phospho- and dephosphopeptide affinity columns.

Immunogen: Phosphopeptide corresponding to amino acid residues surrounding the phospho-Thr180 and phospho-Tyr182 of rat p38 MAPK.

Isotyp: IgG

Anwendungsinformationen

Applikationshinweise: Western blot: 1/1000. Immunohistochemistry: 1/250-1/500.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Buffer: 10 mM HEPES ( pH 7.5), 150 mM NaCl, 100 μg/mL BSA and 50 % Glycerol.

Handhabung: Avoid repeated freezing and thawing.

Lagerung: -20 °C

Informationen zur Lagerung: Store the antibody undiluted (in aliquots) at-20 °C.

Referenzen für anti-MAPK14 Antikörper (ABIN372694)

Chaudhuri, Ghate, Singh, Mandal: "Methyl gallate isolated from Spondias pinnata exhibits anticancer activity against human glioblastoma by induction of apoptosis and sustained extracellular signal-regulated kinase 1/2 activation." in: Pharmacognosy magazine, Vol. 11, Issue 42, pp. 269-76, (2015) (PubMed).

Details zu MAPK14

Target: MAPK14 (Mitogen-Activated Protein Kinase 14 (MAPK14))

Andere Bezeichnung: MAP Kinase p38 alpha / MAPK14

Hintergrund: The three Mitogen-Activated Protein Kinases (MAPKs) are evolutionarily conserved protein kinases that control a vast array of cellular processes. p38 MAPK is one of these kinases and it is activated by both inflammatory cytokines and by stress (Johnson and Lapadat, 2002, Shi and Gaestel, 2002). The p38 MAPK is thought to be particularly important in diseases like asthma and autoimmunity but it also plays important roles in the stress response of the nervous system (Philip and Armstead, 2003, Ying et al., 2002). Like the other MAPKs, p38 is activated by a dual specificity kinase that phosphorylates Thr180 and Tyr182 (Lin et al., 1995).Synonyms: CSBP, CSBP1, CSBP2, CSPB1, Cytokine suppressive anti-inflammatory drug-binding protein, MAX-interacting protein 2, MXI2, Mitogen-activated protein kinase 14, SAPK2A, p38 alpha

Gen-ID: 1432

NCBI Accession: NP_001306

UniProt: Q16539

Pathways: MAPK Signalweg, Neurotrophin Signalübertragung, Activation of Innate immune Response, Cellular Response to Molecule of Bacterial Origin, Regulation of Muscle Cell Differentiation, Regulation of Cell Size, Hepatitis C, Toll-Like Receptors Cascades, Autophagie, Thromboxane A2 Receptor Signaling, BCR Signaling, S100 Proteine